CPLM-012228

Genbank Nucleotide ID

Nuclear transcription factor Y subunit gamma

Protein Synonyms/Alias

CAAT box DNA-binding protein subunit C; Nuclear transcription factor Y subunit C; NF-YC; Transactivator HSM-1/2

Homo sapiens (Human)

Position	Peptide	Type	References
36	EIRNLTVKDFRVQEL	ubiquitination	[1, 2, 3, 4, 5, 6]
53	ARIKKIMKLDEDVKM	ubiquitination	[2, 5]
59	MKLDEDVKMISAEAP	ubiquitination	[2, 5]
92	WIHTEDNKRRTLQRN	ubiquitination	[5]
124	IVPRDELKPPKRQEE	ubiquitination	[5]
127	RDELKPPKRQEEVRQ	ubiquitination	[5]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.

3D-structure; Activator; Alternative splicing; Complete proteome; DNA-binding; Nucleus; Polymorphism; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016602; C:CCAAT-binding factor complex; IDA:UniProtKB.
GO:0003677; F:DNA binding; IDA:UniProtKB.
GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
GO:0006457; P:protein folding; TAS:ProtInc.
GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
GO:0044281; P:small molecule metabolic process; TAS:Reactome.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR003958; CBFA_NFYB_domain.
IPR009072; Histone-fold.
IPR027170; NFYC/HAP5_su.

PF00808; CBFD_NFYB_HMF