CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006593
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription initiation factor IIE subunit alpha 
Protein Synonyms/Alias
 TFIIE-alpha; Factor A 66 kDa subunit; Transcription factor A large subunit 
Gene Name
 TFA1 
Gene Synonyms/Alias
 YKL028W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
90ERVYYYVKYPHAIDAacetylation[1]
460SGPSANAKPNDGDDDubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase (By similarity). 
Sequence Annotation
 DOMAIN 9 99 HTH TFE/IIEalpha-type.
 ZN_FING 124 152 C4-type (Potential).  
Keyword
 Complete proteome; Direct protein sequencing; Metal-binding; Nucleus; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 482 AA 
Protein Sequence
MDRPIDDIVK NLLKFVVRGF YGGSFVLVLD AILFHSVLAE DDLKQLLSIN KTELGPLIAR 60
LRSDRLISIH KQREYPPNSK SVERVYYYVK YPHAIDAIKW KVHQVVQRLK DDLDKNSEPN 120
GYMCPICLTK YTQLEAVQLL NFDRTEFLCS LCDEPLVEDD SGKKNKEKQD KLNRLMDQIQ 180
PIIDSLKKID DSRIEENTFE IALARLIPPQ NQSHAAYTYN PKKGSTMFRP GDSAPLPNLM 240
GTALGNDSSR RAGANSQATL HINITTASDE VAQRELQERQ AEEKRKQNAV PEWHKQSTIG 300
KTALGRLDNE EEFDPVVTAS AMDSINPDNE PAQETSYQNN RTLTEQEMEE RENEKTLNDY 360
YAALAKKQAK LNKEEEEEEE EEEDEEEEEE EEMEDVMDDN DETARENALE DEFEDVTDTA 420
GTAKTESNTS NDVKQESIND KTEDAVNATA TASGPSANAK PNDGDDDDDD DDDEMDIEFE 480
DV 482 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005673; C:transcription factor TFIIE complex; IDA:SGD.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0001113; P:transcriptional open complex formation at RNA polymerase II promoter; IDA:SGD. 
Interpro
 IPR017919; TFIIE/TFIIEa_HTH.
 IPR002853; TFIIE_asu.
 IPR024550; TFIIEa/SarR/Rpc3_HTH_dom.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR013137; Znf_TFIIB. 
Pfam
 PF08271; TF_Zn_Ribbon
 PF02002; TFIIE_alpha 
SMART
 SM00531; TFIIE 
PROSITE
 PS51344; HTH_TFE_IIE 
PRINTS