UniProt Accession

 GLYA_MYCS2; A0R2V7; I7FJP8 

Genbank Protein ID

 CP000480; CP001663 

Genbank Nucleotide ID

 ABK71001.1; AFP41555.1 

Protein Name

 Serine hydroxymethyltransferase 

Protein Synonyms/Alias

 SHMT; Serine methylase 

Gene Name

 glyA 

Gene Synonyms/Alias

 MSMEG_5249; MSMEI_5111 

Created Date

 July 27, 2013 

Organism

 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 

NCBI Taxa ID

 246196 

Lysine Modification

Position	Peptide	Type	References
362	ALADGFVKRDAGLVT	pupylation	[1, 2]

Reference

 [1] Expansion of the mycobacterial "PUPylome".
 Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC.
 Mol Biosyst. 2010 Feb;6(2):376-85. [PMID: 20094657]
 [2] Proteome-wide identification of mycobacterial pupylation targets.
 Poulsen C, Akhter Y, Jeon AH, Schmitt-Ulms G, Meyer HE, Stefanski A, Stühler K, Wilmanns M, Song YH.
 Mol Syst Biol. 2010 Jul 13;6:386. [PMID: 20631680] 

Functional Description

 Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism (By similarity). 

Sequence Annotation

 REGION 183 185 Substrate binding (By similarity).
 BINDING 62 62 Pyridoxal phosphate (By similarity).
 BINDING 82 82 Pyridoxal phosphate (By similarity).
 BINDING 84 84 Substrate (By similarity).
 BINDING 91 91 Substrate (By similarity).
 BINDING 92 92 Pyridoxal phosphate (By similarity).
 BINDING 179 179 Substrate; via carbonyl oxygen (By
 BINDING 234 234 Pyridoxal phosphate (By similarity).
 BINDING 262 262 Pyridoxal phosphate (By similarity).
 BINDING 290 290 Pyridoxal phosphate (By similarity).
 BINDING 297 297 Pyridoxal phosphate (By similarity).
 BINDING 321 321 Pyridoxal phosphate; via amide nitrogen
 BINDING 421 421 Pyridoxal phosphate (By similarity).
 MOD_RES 291 291 N6-(pyridoxal phosphate)lysine (By
 CROSSLNK 362 362 Isoglutamyl lysine isopeptide (Lys-Gln)  

Keyword

 Amino-acid biosynthesis; Complete proteome; Cytoplasm; Isopeptide bond; One-carbon metabolism; Pyridoxal phosphate; Reference proteome; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 490 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004372; F:glycine hydroxymethyltransferase activity; IEA:HAMAP.
 GO:0030170; F:pyridoxal phosphate binding; IEA:HAMAP.
 GO:0019264; P:glycine biosynthetic process from serine; IEA:HAMAP.
 GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. 

Interpro

 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2.
 IPR001085; Ser_HO-MeTrfase.
 IPR019798; Ser_HO-MeTrfase_PLP_BS. 

Pfam

 PF00464; SHMT 

SMART

  

PROSITE

 PS00096; SHMT 

PRINTS