| Tag | Content |
|---|
| CPLM ID | CPLM-016038 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Serine/threonine-protein kinase WNK4 |
Protein Synonyms/Alias | Protein kinase lysine-deficient 4; Protein kinase with no lysine 4 |
Gene Name | Wnk4 |
Gene Synonyms/Alias | Prkwnk4 |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 396 | TKPNSFYKVKMPEVK | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. |
Sequence Annotation | DOMAIN 171 429 Protein kinase.
NP_BIND 177 185 ATP (By similarity).
ACT_SITE 299 299 Proton acceptor (By similarity).
BINDING 183 183 ATP (By similarity).
MOD_RES 328 328 Phosphoserine; by autocatalysis (By
MOD_RES 332 332 Phosphoserine; by autocatalysis (By |
Keyword | ATP-binding; Cell junction; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Tight junction; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1222 AA |
Protein Sequence | MLAPRNTETG VPMSQTEADL ALRPSPALTS TGPTRLGPPP RRVRRFSGKA EPRPRSSRPS 60
RRSSVDLGLL SSWSQPASLL PEPPDPPDSA GPTRSPPSSS KEPPEGTWMG AAPVKAVDSA 120
CPELTGSSGG PGSREPPRVP DAAARERRRE QEEKEDTETQ AVATSPDGRY LKFDIEIGRG 180
SFKTVYRGLD TDTTVEVAWC ELQTRKLSRA ERQRFSEEVE MLKGLQHPNI VRFYDSWKSV 240
LRGQVCIVLV TELMTSGTLK TYLRRFREMK PRVLQRWSRQ ILRGLHFLHS RVPPILHRDL 300
KCDNVFITGP SGSVKIGDLG LATLKRASFA KSVIGTPEFM APEMYEEKYD EAVDVYAFGM 360
CMLEMATSEY PYSECQNAAQ IYRKVTSGTK PNSFYKVKMP EVKEIIEGCI RTDKNERFTI 420
QDLLAHAFFR EERGVHVELA EEDDGEKPGL KLWLRMEDAR RGGRPRDNQA IEFLFQLGRD 480
AAEEVAQEMV ALGLVCEADY QPVARAVRER VAAIQRKREK LRKARELEVL PPDSGPPPAT 540
VSLAPGPPSA FPPEPEEPEA DQHQSFLFRH ASYSSTTSDC ETDGYLSSSG FLDASDPALQ 600
PPGGLPSSPA ESHLCLPSGF ALSIPRSGPG SDFSPGDSYA SDAASGLSDM GEGGQMRKNP 660
VKTLRRRPRS RLRVTSVSDQ SDRVVECQLQ THNSKMVTFR FDLDGDSPEE IAAAMVYNEF 720
ILPSERDGFL SRIREIIQRV ETLLKRDAGP PEAAEDALSP QEEPAALPAL PGPPNAEPQR 780
SISPEQRSWA AFSTSPSSPG TPLSPGAPFS PGTPPVFPCP IFPITSPSCY PCPFSQVSSN 840
PYPQAPSSLL PLSSSASQVP LPSSSLPISA PLPFSPSYPQ DPLSPTSLPV CPSPPSLPST 900
TAAPLLSLAS AFSLAVMTVA QSLLSPSPGL LSQSPPAPPG PLPSLPLSLA SCDQESLSAQ 960
TAETENEASR NPAQPLLGDA RLAPISEEGK PQLVGRFQVT SSKEPAEPPL QPASPTLSRS 1020
LKLPSPPLTS ESSDTEDSAA GGPETREALA ESDRAAEGLG VAVDDEKDEG KEPLLGGSSP 1080
ILSHPSPVWM NYSYSSLCLS SEESESSGED EEFWAELQNL RQKHLSEVEA LQTLQKKEIE 1140
DLYSRLGKQP PPGIVAPAAM LSCRQRRLSK GSFPTSRRNS LQRSDLPGPG IMRRNSLSGS 1200
STGSQEQRAS KGVTFAGDIG RM 1222 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:MGI. GO:0005923; C:tight junction; IDA:UniProtKB. GO:0005524; F:ATP binding; ISS:UniProtKB. GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB. GO:0030644; P:cellular chloride ion homeostasis; TAS:MGI. GO:0006821; P:chloride transport; IDA:MGI. GO:0007243; P:intracellular protein kinase cascade; ISS:UniProtKB. GO:0090188; P:negative regulation of pancreatic juice secretion; IMP:MGI. GO:0008104; P:protein localization; IDA:MGI. |
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