| Tag | Content |
|---|
| CPLM ID | CPLM-004207 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | ARS-binding factor 1 |
Protein Synonyms/Alias | Bidirectionally acting factor 1; DNA replication enhancer-binding protein OBF1; SFB-B |
Gene Name | ABF1 |
Gene Synonyms/Alias | BAF1; OBF1; REB2; SBF1; YKL112W; YKL505 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 18 | YKHPIINKDLAIGAH | ubiquitination | [1] | | 383 | NSNHTVSKFKVSHYV | acetylation | [2] | | 487 | GTSSNTTKNVNNNKN | acetylation | [2] | | 518 | LMESVLDKTSSHRYQ | acetylation | [2] | | 584 | TAQLAIDKINSYKRS | acetylation | [2] |
|
Reference | [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae. Starita LM, Lo RS, Eng JK, von Haller PD, Fields S. Proteomics. 2012 Jan;12(2):236-40. [ PMID: 22106047] [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | General regulatory factor (GRF) that contributes to transcriptional activation of a large number of genes, as well as to DNA replication, silencing and telomere structure. Involved in the transcription activation of a subset of ribosomal protein genes. Binds the ARS-elements found in many promoters. Binds to the sequence 5'-TCN(7)ACG-3'. Influences on genome-wide nucleosome occupancy and affects chromatin structure, and probably dynamics. As a component of the global genome repair (GGR) complex, promotes global genome nucleotide excision repair (GG-NER) which removes DNA damage from nontranscribing DNA. Component of the regulatory network controlling mitotic and meiotic cell cycle progression. |
Sequence Annotation | REGION 624 628 C-terminal sequence 1.
REGION 639 662 C-terminal sequence 2.
MOD_RES 189 189 Phosphothreonine.
MOD_RES 193 193 Phosphoserine.
MOD_RES 467 467 Phosphoserine.
MOD_RES 554 554 Phosphoserine.
MOD_RES 618 618 Phosphoserine.
MOD_RES 624 624 Phosphoserine; by PKC (Potential).
MOD_RES 720 720 Phosphoserine; by CK2. |
Keyword | Activator; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA replication; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 731 AA |
Protein Sequence | MDKLVVNYYE YKHPIINKDL AIGAHGGKKF PTLGAWYDVI NEYEFQTRCP IILKNSHRNK 60
HFTFACHLKN CPFKVLLSYA GNAASSETSS PSANNNTNPP GTPDHIHHHS NNMNNEDNDN 120
NNGSNNKVSN DSKLDFVTDD LEYHLANTHP DDTNDKVESR SNEVNGNNDD DADANNIFKQ 180
QGVTIKNDTE DDSINKASID RGLDDESGPT HGNDSGNHRH NEEDDVHTQM TKNYSDVVND 240
EDINVAIANA VANVDSQSNN KHDGKDDDAT NNNDGQDNNT NNDHNNNSNI NNNNVGSHGI 300
SSHSPSSIRD TSMNLDVFNS ATDDIPGPFV VTKIEPYHSH PLEDNLSLGK FILTKIPKIL 360
QNDLKFDQIL ESSYNNSNHT VSKFKVSHYV EESGLLDILM QRYGLTAEDF EKRLLSQIAR 420
RITTYKARFV LKKKKMGEYN DLQPSSSSNN NNNNDGELSG TNLRSNSIDY AKHQEISSAG 480
TSSNTTKNVN NNKNDSNDDN NGNNNNDASN LMESVLDKTS SHRYQPKKMP SVNKWSKPDQ 540
ITHSDVSMVG LDESNDGGNE NVHPTLAEVD AQEARETAQL AIDKINSYKR SIDDKNGDGH 600
NNSSRNVVDE NLINDMDSED AHKSKRQHLS DITLEERNED DKLPHEVAEQ LRLLSSHLKE 660
VENLHQNNDD DVDDVMVDVD VESQYNKNTT HHNNHHSQPH HDEEDVAGLI GKADDEEDLS 720
DENIQPELRG Q 731 |
Gene Ontology | GO:0000113; C:nucleotide-excision repair factor 4 complex; IDA:SGD. GO:0003688; F:DNA replication origin binding; IDA:SGD. GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IDA:SGD. GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:SGD. GO:0044374; F:sequence-specific DNA binding, bending; IDA:SGD. GO:0006338; P:chromatin remodeling; IDA:SGD. GO:0030466; P:chromatin silencing at silent mating-type cassette; IMP:SGD. GO:0006261; P:DNA-dependent DNA replication; IDA:SGD. GO:0070911; P:global genome nucleotide-excision repair; IDA:SGD. GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:SGD. |
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