UniProt Accession

 PYRE_YEAST; P13298; D6W0H8 

Genbank Protein ID

 X14795; Z49210; AY693160; X65783; BK006946 

Genbank Nucleotide ID

 CAA32901.1; CAA89112.1; AAT93179.1; CAA46665.1; DAA09792.1 

Protein Name

 Orotate phosphoribosyltransferase 1 

Protein Synonyms/Alias

 OPRT 1; OPRTase 1 

Gene Name

 URA5 

Gene Synonyms/Alias

 PYR5; YML106W; YM8339.13 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
28	ALRFGSFKLKSGRES	ubiquitination	[1]
110	AFNRKEAKDHGEGGI	acetylation	[2]
173	EVVSTDDKEGLSATQ	acetylation	[2]
173	EVVSTDDKEGLSATQ	ubiquitination	[1]
214	ITAEEKSKIEQYLQT	acetylation	[2]
214	ITAEEKSKIEQYLQT	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) (By similarity). 

Sequence Annotation

 REGION 38 39 Orotate binding (By similarity).
 REGION 76 77 5-phosphoribose 1-diphosphate binding (By
 REGION 132 140 5-phosphoribose 1-diphosphate binding (By
 BINDING 30 30 5-phosphoribose 1-diphosphate (By
 BINDING 106 106 5-phosphoribose 1-diphosphate; shared
 BINDING 107 107 5-phosphoribose 1-diphosphate (By
 BINDING 110 110 5-phosphoribose 1-diphosphate; shared
 BINDING 112 112 5-phosphoribose 1-diphosphate; shared
 BINDING 136 136 Orotate (By similarity).
 BINDING 164 164 Orotate (By similarity).
 MOD_RES 213 213 Phosphoserine.
 MOD_RES 225 225 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Glycosyltransferase; Phosphoprotein; Pyrimidine biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 226 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0004588; F:orotate phosphoribosyltransferase activity; IDA:SGD.
 GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IMP:SGD.
 GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0046132; P:pyrimidine ribonucleoside biosynthetic process; IDA:SGD. 

Interpro

 IPR023031; OPRT.
 IPR004467; Or_phspho_trans_dom.
 IPR000836; PRibTrfase_dom. 

Pfam

 PF00156; Pribosyltran 

SMART

  

PROSITE

 PS00103; PUR_PYR_PR_TRANSFER 

PRINTS