CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011122
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calpain-like protease 1 
Protein Synonyms/Alias
 Calpain-7; Cysteine protease RIM13; Regulator of IME2 protein 13 
Gene Name
 RIM13 
Gene Synonyms/Alias
 CPL1; YMR154C; YM8520.03C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
140SLINLRSKNLNLPLIacetylation[1]
183IPTSVDGKQLSLKSNubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Required for the proteolytic cleavage of the transcriptional repressor RIM101 in response to alkaline ambient pH, which is necessary for sporulation and invasive growth. Probably the protease that cleaves RIM101. 
Sequence Annotation
 DOMAIN 70 317 Calpain catalytic.
 ACT_SITE 128 128 By similarity.
 ACT_SITE 271 271 By similarity.
 ACT_SITE 296 296 By similarity.  
Keyword
 Complete proteome; Hydrolase; Protease; Reference proteome; Thiol protease. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 727 AA 
Protein Sequence
MNDWHEFNAA IKSIYCNAEG DSSSIINRLV GLAMKSEDST FIEAVLVLKE NVSKVDKQLR 60
FLWLTSTINS RFYPPIPISE ASPVSWNKTE YCAPGTEELQ RRYPGRAKLQ NEEDYSGGIE 120
QCRDVPDCSL VASLINLRSK NLNLPLIKQI SSTKYHVNLS FNGSNKRLVT VDISQIPTSV 180
DGKQLSLKSN DISDKIGELA LLLVSKGTYS TDGSNISIDT YRLSGFLPEI TQVNSYPFEK 240
LWKFHKSNLC LMGAGTGNRS NDMIKPLVEN HDYSIIDITY DSRLVKLRDP RNSALNVEIS 300
YEQYLKNFKQ LYLNWNQEKL FKRSQVLHFR YDTSRYNKFS IVADKPLFHL VNNSKVTETV 360
WLLLESHLQD EGSQENRSVS FLNEAPECII CPIEPPVECG GNHIGLQLVK LRLDAETERL 420
LYCYSTTNNN FSIHSFSVVK EICFQRLKDT KSLFAKVLFS FPYEIEGKAS FDTCNFFQNP 480
TFELEVHSEQ DYQVLMDAAC ISTSSHDLIN IQVYYFNDYE LIKPIMFDNH YQPGQGLKQD 540
VPILTNVKYM IVCSTYGPPA STEFELLASI RLSSSWRLIS GITLRSVNLI YGTYPYHCRN 600
RFHWKETSDK LKIQMTLPTK KYSTNKLFIR VVPVESSARL RMRCNIFEPE SALCVYECQE 660
YRTCPSGGIV IPDLEVSRTN IVVLMIERSV PISSCLPTEG QLDELELFVG SSQKIRIEKY 720
SDDVIPK 727 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro.
 GO:0004197; F:cysteine-type endopeptidase activity; IDA:SGD.
 GO:0016485; P:protein processing; IGI:SGD.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR001300; Peptidase_C2_calpain_cat. 
Pfam
  
SMART
 SM00230; CysPc 
PROSITE
 PS50203; CALPAIN_CAT 
PRINTS