UniProt Accession

 PURA_MOUSE; P42669 

Genbank Protein ID

 U02098; AF017631 

Genbank Nucleotide ID

 AAA64630.1; AAB71860.1 

Protein Name

 Transcriptional activator protein Pur-alpha 

Protein Synonyms/Alias

 Purine-rich single-stranded DNA-binding protein alpha 

Gene Name

 Pura 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
77	KRFYLDVKQNAKGRF	ubiquitination	[1]
86	NAKGRFLKIAEVGAG	acetylation	[2]
86	NAKGRFLKIAEVGAG	ubiquitination	[1]
96	EVGAGGNKSRLTLSM	acetylation	[3]
96	EVGAGGNKSRLTLSM	succinylation	[3]
159	RKYYMDLKENQRGRF	ubiquitination	[1]
238	FFDVGSNKYGVFMRV	ubiquitination	[1]
261	NSITVPYKVWAKFGH	ubiquitination	[1]
265	VPYKVWAKFGHTFCK	acetylation	[4]
272	KFGHTFCKYSEEMKK	acetylation	[4, 5, 6, 7]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the c-Myc gene. May play a role in the initiation of DNA replication and in recombination. 

Sequence Annotation

 MOD_RES 252 252 Phosphotyrosine.  

Keyword

 Activator; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 321 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0030425; C:dendrite; IDA:MGI.
 GO:0005662; C:DNA replication factor A complex; IDA:UniProtKB.
 GO:0043025; C:neuronal cell body; IDA:MGI.
 GO:0003690; F:double-stranded DNA binding; IDA:MGI.
 GO:0003691; F:double-stranded telomeric DNA binding; IEA:Compara.
 GO:0032422; F:purine-rich negative regulatory element binding; IDA:HGNC.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:MGI.
 GO:0003697; F:single-stranded DNA binding; IDA:UniProtKB.
 GO:0046332; F:SMAD binding; IDA:MGI.
 GO:0000900; F:translation repressor activity, nucleic acid binding; IDA:HGNC.
 GO:0006915; P:apoptotic process; NAS:UniProtKB.
 GO:0030154; P:cell differentiation; NAS:UniProtKB.
 GO:0008283; P:cell proliferation; NAS:UniProtKB.
 GO:0006268; P:DNA unwinding involved in replication; IEA:Compara.
 GO:0007093; P:mitotic cell cycle checkpoint; NAS:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:HGNC.
 GO:0007399; P:nervous system development; IMP:MGI.
 GO:0008284; P:positive regulation of cell proliferation; IMP:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR006628; PUR_DNA_RNA-bd. 

Pfam

 PF04845; PurA 

SMART

 SM00712; PUR 

PROSITE

  

PRINTS