UniProt Accession

 OLA1_YEAST; P38219; D6VQ27 

Genbank Protein ID

 X76078; Z35894; BK006936 

Genbank Nucleotide ID

 CAA53682.1; CAA84967.1; DAA07147.1 

Protein Name

 Obg-like ATPase 1 

Protein Synonyms/Alias

  

Gene Name

 OLA1 

Gene Synonyms/Alias

 YBR025C; YBR0309 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
22	GRPGNNLKAGIVGLA	ubiquitination	[1]
33	VGLANVGKSTFFQAI	ubiquitination	[1]
79	KLCEIYKKTASEVPA	ubiquitination	[1]
98	YDIAGLTKGASAGEG	ubiquitination	[2]
155	INQELRLKDIEFAQK	acetylation	[3]
155	INQELRLKDIEFAQK	ubiquitination	[1]
162	KDIEFAQKALEGAEK	acetylation	[3]
169	KALEGAEKIAKRGGQ	ubiquitination	[1]
192	EEMDLITKIIKLLES	acetylation	[3]
195	DLITKIIKLLESGQR	acetylation	[3]
195	DLITKIIKLLESGQR	ubiquitination	[1]
286	EDAEEELKKLQTISA	acetylation	[3]
296	QTISALPKIITTMRQ	acetylation	[3]
296	QTISALPKIITTMRQ	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP. 

Sequence Annotation

 DOMAIN 23 129 G.
 NP_BIND 30 35 ATP (By similarity).
 BINDING 233 233 ATP; via carbonyl oxygen (By similarity).
 MOD_RES 89 89 Phosphothreonine.
 MOD_RES 116 116 Phosphoserine.
 MOD_RES 119 119 Phosphoserine.  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Nucleotide-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 394 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IDA:SGD.
 GO:0005525; F:GTP binding; IEA:InterPro. 

Interpro

 IPR012675; Beta-grasp_dom.
 IPR004396; CHP00092.
 IPR013029; DUF933.
 IPR006073; GTP_binding_domain.
 IPR027417; P-loop_NTPase.
 IPR012676; TGS-like.
 IPR023192; TGS-like_dom. 

Pfam

 PF01926; MMR_HSR1
 PF06071; YchF-GTPase_C 

SMART

  

PROSITE

  

PRINTS

 PR00326; GTP1OBG.