CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012053
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Voltage-gated potassium channel subunit beta-2 
Protein Synonyms/Alias
 K(+) channel subunit beta-2; Kv-beta-2; hKvbeta2 
Gene Name
 KCNAB2 
Gene Synonyms/Alias
 KCNA2B; KCNK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
124TKIFWGGKAETERGLacetylation[1]
124TKIFWGGKAETERGLubiquitination[2, 3]
224YHMFQREKVEVQLPEubiquitination[2, 3]
268PYSRASLKGYQWLKDubiquitination[2, 3, 4, 5]
276GYQWLKDKILSEEGRacetylation[3]
276GYQWLKDKILSEEGRubiquitination[2]
290RRQQAKLKELQAIAEubiquitination[2, 3, 4]
358IDSILGNKPYSKKDYubiquitination[2]
362LGNKPYSKKDYRS**acetylation[1]
362LGNKPYSKKDYRS**ubiquitination[2]
363GNKPYSKKDYRS***ubiquitination[2, 3]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit. Alters functional properties of Kv1.4. 
Sequence Annotation
 NP_BIND 56 57 NADP.
 NP_BIND 188 189 NADP.
 NP_BIND 243 248 NADP.
 NP_BIND 323 329 NADP.
 BINDING 63 63 NADP.
 BINDING 85 85 NADP.
 BINDING 90 90 NADP.
 BINDING 214 214 NADP.
 BINDING 254 254 NADP.
 MOD_RES 9 9 Phosphoserine.
 MOD_RES 20 20 Phosphoserine (By similarity).
 MOD_RES 25 25 Phosphotyrosine (By similarity).
 MOD_RES 124 124 N6-acetyllysine.
 MOD_RES 360 360 Phosphotyrosine (By similarity).  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Ion channel; Ion transport; NADP; Phosphoprotein; Polymorphism; Potassium; Potassium transport; Reference proteome; Transport; Voltage-gated channel. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 367 AA 
Protein Sequence
MYPESTTGSP ARLSLRQTGS PGMIYSTRYG SPKRQLQFYR NLGKSGLRVS CLGLGTWVTF 60
GGQITDEMAE QLMTLAYDNG INLFDTAEVY AAGKAEVVLG NIIKKKGWRR SSLVITTKIF 120
WGGKAETERG LSRKHIIEGL KASLERLQLE YVDVVFANRP DPNTPMEETV RAMTHVINQG 180
MAMYWGTSRW SSMEIMEAYS VARQFNLTPP ICEQAEYHMF QREKVEVQLP ELFHKIGVGA 240
MTWSPLACGI VSGKYDSGIP PYSRASLKGY QWLKDKILSE EGRRQQAKLK ELQAIAERLG 300
CTLPQLAIAW CLRNEGVSSV LLGASNADQL MENIGAIQVL PKLSSSIIHE IDSILGNKPY 360
SKKDYRS 367 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0044224; C:juxtaparanode region of axon; ISS:BHF-UCL.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0015459; F:potassium channel regulator activity; TAS:ProtInc.
 GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0007268; P:synaptic transmission; TAS:Reactome. 
Interpro
 IPR001395; Aldo/ket_red.
 IPR005983; K_chnl_volt-dep_bsu_KCNAB.
 IPR005399; K_chnl_volt-dep_bsu_KCNAB-rel.
 IPR005401; K_chnl_volt-dep_bsu_KCNAB2.
 IPR023210; NADP_OxRdtase_dom. 
Pfam
 PF00248; Aldo_ket_red 
SMART
  
PROSITE
  
PRINTS
 PR01579; KCNAB2CHANEL.
 PR01577; KCNABCHANNEL.