CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005099
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcriptional regulatory protein SIN3 
Protein Synonyms/Alias
  
Gene Name
 SIN3 
Gene Synonyms/Alias
 CPE1; GAM2; RPD1; SDI1; SDS16; UME4; YOL004W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
253LDIMKDFKSQAIDTPubiquitination[1]
679AKRYIGNKHLYTEFLacetylation[2]
729NFVGYQEKTKCIENIacetylation[2]
846ENMTENEKANFKLPPubiquitination[1]
1170QMNERVTKEINTRSTubiquitination[1]
1354TEGIPHEKLKIPFLEacetylation[2]
1356GIPHEKLKIPFLERLubiquitination[1]
1417FTRKATNKYPTIANDacetylation[2]
1428IANDNTQKGMVSQKKubiquitination[1]
1440QKKELISKFLDCAVGacetylation[2]
1440QKKELISKFLDCAVGubiquitination[1]
1457NNLDEAQKLSMQKKWubiquitination[1]
1473NLKDSIAKTSAGNQGubiquitination[1]
1487GIESETEKGKITKQEacetylation[2]
1531GNTESSDKGAKIQ**acetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Catalytic component of the RPD3 histone deacetylase complexes RPD3C(L) and RPD3C(S) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. SIN3 has also a RPD3 independent function required for normal longevity. 
Sequence Annotation
 DOMAIN 217 287 PAH 1.
 DOMAIN 404 474 PAH 2.
 DOMAIN 656 727 PAH 3.
 MOD_RES 137 137 Phosphoserine.
 MOD_RES 303 303 Phosphothreonine.
 MOD_RES 304 304 Phosphothreonine.
 MOD_RES 316 316 Phosphoserine.
 MOD_RES 1046 1046 Phosphoserine.  
Keyword
 Activator; Cell cycle; Cell division; Chromatin regulator; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1536 AA 
Protein Sequence
MSQVWHNSNS QSNDVATSND ATGSNERNEK EPSLQGNKPG FVQQQQRITL PSLSALSTKE 60
EDRRDSNGQQ ALTSHAAHIL GYPPPHSNAM PSIATDSALK QPHEYHPRPK SSSSSPSINA 120
SLMNAGPAPL PTVGAASFSL SRFDNPLPIK APVHTEEPKS YNGLQEEEKA TQRPQDCKEV 180
PAGVQPADAP DPSSNHADAN DDNNNNENSH DEDADYRPLN VKDALSYLEQ VKFQFSSRPD 240
IYNLFLDIMK DFKSQAIDTP GVIERVSTLF RGYPILIQGF NTFLPQGYRI ECSSNPDDPI 300
RVTTPMGTTT VNNNISPSGR GTTDAQELGS FPESDGNGVQ QPSNVPMVPS SVYQSEQNQD 360
QQQSLPLLAT SSGLPSIQQP EMPAHRQIPQ SQSLVPQEDA KKNVDVEFSQ AISYVNKIKT 420
RFADQPDIYK HFLEILQTYQ REQKPINEVY AQVTHLFQNA PDLLEDFKKF LPDSSASANQ 480
QVQHAQQHAQ QQHEAQMHAQ AQAQAQAQAQ VEQQKQQQQF LYPASGYYGH PSNRGIPQQN 540
LPPIGSFSPP TNGSTVHEAY QDQQHMQPPH FMPLPSIVQH GPNMVHQGIA NENPPLSDLR 600
TSLTEQYAPS SIQHQQQHPQ SISPIANTQY GDIPVRPEID LDPSIVPVVP EPTEPIENNI 660
SLNEEVTFFE KAKRYIGNKH LYTEFLKILN LYSQDILDLD DLVEKVDFYL GSNKELFTWF 720
KNFVGYQEKT KCIENIVHEK HRLDLDLCEA FGPSYKRLPK SDTFMPCSGR DDMCWEVLND 780
EWVGHPVWAS EDSGFIAHRK NQYEETLFKI EEERHEYDFY IESNLRTIQC LETIVNKIEN 840
MTENEKANFK LPPGLGHTSM TIYKKVIRKV YDKERGFEII DALHEHPAVT APVVLKRLKQ 900
KDEEWRRAQR EWNKVWRELE QKVFFKSLDH LGLTFKQADK KLLTTKQLIS EISSIKVDQT 960
NKKIHWLTPK PKSQLDFDFP DKNIFYDILC LADTFITHTT AYSNPDKERL KDLLKYFISL 1020
FFSISFEKIE ESLYSHKQNV SESSGSDDGS SIASRKRPYQ QEMSLLDILH RSRYQKLKRS 1080
NDEDGKVPQL SEPPEEEPNT IEEEELIDEE AKNPWLTGNL VEEANSQGII QNRSIFNLFA 1140
NTNIYIFFRH WTTIYERLLE IKQMNERVTK EINTRSTVTF AKDLDLLSSQ LSEMGLDFVG 1200
EDAYKQVLRL SRRLINGDLE HQWFEESLRQ AYNNKAFKLY TIDKVTQSLV KHAHTLMTDA 1260
KTAEIMALFV KDRNASTTSA KDQIIYRLQV RSHMSNTENM FRIEFDKRTL HVSIQYIALD 1320
DLTLKEPKAD EDKWKYYVTS YALPHPTEGI PHEKLKIPFL ERLIEFGQDI DGTEVDEEFS 1380
PEGISVSTLK IKIQPITYQL HIENGSYDVF TRKATNKYPT IANDNTQKGM VSQKKELISK 1440
FLDCAVGLRN NLDEAQKLSM QKKWENLKDS IAKTSAGNQG IESETEKGKI TKQEQSDNLD 1500
SSTASVLPAS ITTVPQDDNI ETTGNTESSD KGAKIQ 1536 
Gene Ontology
 GO:0033698; C:Rpd3L complex; IDA:SGD.
 GO:0070210; C:Rpd3L-Expanded complex; IDA:SGD.
 GO:0032221; C:Rpd3S complex; IDA:SGD.
 GO:0003713; F:transcription coactivator activity; IMP:SGD.
 GO:0003714; F:transcription corepressor activity; IMP:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0006303; P:double-strand break repair via nonhomologous end joining; IMP:SGD.
 GO:0016575; P:histone deacetylation; IMP:SGD.
 GO:0061188; P:negative regulation of chromatin silencing at rDNA; IMP:SGD.
 GO:0061186; P:negative regulation of chromatin silencing at silent mating-type cassette; IMP:SGD.
 GO:0031939; P:negative regulation of chromatin silencing at telomere; IMP:SGD.
 GO:0051038; P:negative regulation of transcription during meiosis; IMP:SGD.
 GO:0016479; P:negative regulation of transcription from RNA polymerase I promoter; IMP:SGD.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0061408; P:positive regulation of transcription from RNA polymerase II promoter in response to heat stress; IMP:SGD.
 GO:0030174; P:regulation of DNA-dependent DNA replication initiation; IMP:SGD.
 GO:0000117; P:regulation of transcription involved in G2/M-phase of mitotic cell cycle; IGI:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR013194; HDAC_interact.
 IPR003822; PAH. 
Pfam
 PF02671; PAH
 PF08295; Sin3_corepress 
SMART
 SM00761; HDAC_interact 
PROSITE
 PS51477; PAH 
PRINTS