UniProt Accession

 GLYA_ECODH; B1XB26 

Genbank Protein ID

 CP000948 

Genbank Nucleotide ID

 ACB03703.1 

Protein Name

 Serine hydroxymethyltransferase 

Protein Synonyms/Alias

 SHMT; Serine methylase 

Gene Name

 glyA 

Gene Synonyms/Alias

 ECDH10B_2718 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12 / DH10B) 

NCBI Taxa ID

 316385 

Lysine Modification

Position	Peptide	Type	References
62	YAEGYPGKRYYGGCE	succinylation	[1]
242	RGGLILAKGGSEELY	succinylation	[1]
250	GGSEELYKKLNSAVF	succinylation	[1]
277	AGKAVALKEAMEPEF	succinylation	[1]
293	TYQQQVAKNAKAMVE	succinylation	[1]
331	VDKNLTGKEADAALG	succinylation	[1]
346	RANITVNKNSVPNDP	succinylation	[1]
354	NSVPNDPKSPFVTSG	succinylation	[1]

Reference

 [1] Identification of lysine succinylation as a new post-translational modification.
 Zhang Z, Tan M, Xie Z, Dai L, Chen Y, Zhao Y.
 Nat Chem Biol. 2011 Jan;7(1):58-63. [PMID: 21151122] 

Functional Description

 Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism (By similarity). 

Sequence Annotation

 REGION 125 127 Substrate binding (By similarity).
 REGION 355 357 Substrate binding (By similarity).
 BINDING 35 35 Pyridoxal phosphate (By similarity).
 BINDING 55 55 Pyridoxal phosphate (By similarity).
 BINDING 57 57 Substrate (By similarity).
 BINDING 64 64 Substrate (By similarity).
 BINDING 65 65 Pyridoxal phosphate (By similarity).
 BINDING 99 99 Pyridoxal phosphate (By similarity).
 BINDING 121 121 Substrate; via carbonyl oxygen (By
 BINDING 175 175 Pyridoxal phosphate (By similarity).
 BINDING 203 203 Pyridoxal phosphate (By similarity).
 BINDING 228 228 Pyridoxal phosphate (By similarity).
 BINDING 235 235 Pyridoxal phosphate (By similarity).
 BINDING 263 263 Pyridoxal phosphate; via amide nitrogen
 BINDING 363 363 Pyridoxal phosphate (By similarity).
 MOD_RES 54 54 N6-acetyllysine (By similarity).
 MOD_RES 229 229 N6-(pyridoxal phosphate)lysine (By
 MOD_RES 250 250 N6-acetyllysine (By similarity).
 MOD_RES 285 285 N6-acetyllysine (By similarity).
 MOD_RES 354 354 N6-acetyllysine (By similarity).
 MOD_RES 375 375 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Amino-acid biosynthesis; Complete proteome; Cytoplasm; One-carbon metabolism; Pyridoxal phosphate; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 417 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004372; F:glycine hydroxymethyltransferase activity; IEA:HAMAP.
 GO:0030170; F:pyridoxal phosphate binding; IEA:HAMAP.
 GO:0019264; P:glycine biosynthetic process from serine; IEA:HAMAP.
 GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. 

Interpro

 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2.
 IPR001085; Ser_HO-MeTrfase.
 IPR019798; Ser_HO-MeTrfase_PLP_BS. 

Pfam

 PF00464; SHMT 

SMART

  

PROSITE

 PS00096; SHMT 

PRINTS