CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013975
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase HECTD3 
Protein Synonyms/Alias
 HECT domain-containing protein 3 
Gene Name
 HECTD3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
50VPREVLYKLYKDPAGubiquitination[1]
53EVLYKLYKDPAGPSRubiquitination[1]
308TDDNFMPKRVVVYGGubiquitination[1]
321GGEGDNLKKLSDVSIubiquitination[1, 2]
322GEGDNLKKLSDVSIDubiquitination[1]
467LRDSESSKPSFMPRLubiquitination[1]
705RFIQLVQKARLEESKubiquitination[1]
742TWQELEKKVCGDPEVubiquitination[1]
808RIYIYPDKLGYETTDubiquitination[1, 2]
841SAKVCEEKLRYAAYNubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity). 
Sequence Annotation
 DOMAIN 219 397 DOC.
 DOMAIN 512 857 HECT.
 ACT_SITE 823 823 Glycyl thioester intermediate.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Ligase; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 861 AA 
Protein Sequence
MAGPGPGAVL ESPRQLLGRV RFLAEAARSL RAGRPLPAAL AFVPREVLYK LYKDPAGPSR 60
VLLPVWEAEG LGLRVGAAGP APGTGSGPLR AARDSIELRR GACVRTTGEE LCNGHGLWVK 120
LTKEQLAEHL GDCGLQEGWL LVCRPAEGGA RLVPIDTPNH LQRQQQLFGV DYRPVLRWEQ 180
VVDLTYSHRL GSRPQPAEAY AEAVQRLLYV PPTWTYECDE DLIHFLYDHL GKEDENLGSV 240
KQYVESIDVS SYTEEFNVSC LTDSNADTYW ESDGSQCQHW VRLTMKKGTI VKKLLLTVDT 300
TDDNFMPKRV VVYGGEGDNL KKLSDVSIDE TLIGDVCVLE DMTVHLPIIE IRIVECRDDG 360
IDVRLRGVKI KSSRQRELGL NADLFQPTSL VRYPRLEGTD PEVLYRRAVL LQRFIKILDS 420
VLHHLVPAWD HTLGTFSEIK QVKQFLLLSR QRPGLVAQCL RDSESSKPSF MPRLYINRRL 480
AMEHRACPSR DPACKNAVFT QVYEGLKPSD KYEKPLDYRW PMRYDQWWEC KFIAEGIIDQ 540
GGGFRDSLAD MSEELCPSSA DTPVPLPFFV RTANQGNGTG EARDMYVPNP SCRDFAKYEW 600
IGQLMGAALR GKEFLVLALP GFVWKQLSGE EVSWSKDFPA VDSVLVKLLE VMEGMDKETF 660
EFKFGKELTF TTVLSDQQVV ELIPGGAGIV VGYGDRSRFI QLVQKARLEE SKEQVAAMQA 720
GLLKVVPQAV LDLLTWQELE KKVCGDPEVT VDALRKLTRF EDFEPSDSRV QYFWEALNNF 780
TNEDRSRFLR FVTGRSRLPA RIYIYPDKLG YETTDALPES STCSSTLFLP HYASAKVCEE 840
KLRYAAYNCV AIDTDMSPWE E 861 
Gene Ontology
 GO:0005634; C:nucleus; IBA:RefGenome.
 GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IBA:RefGenome. 
Interpro
 IPR004939; APC_su10/DOC_dom.
 IPR008979; Galactose-bd-like.
 IPR000569; HECT. 
Pfam
 PF03256; APC10
 PF00632; HECT 
SMART
 SM00119; HECTc 
PROSITE
 PS51284; DOC
 PS50237; HECT 
PRINTS