UniProt Accession

 RS7_ECOLI; P02359; Q2M706 

Genbank Protein ID

 U18997; U00096; AP009048; V00355; J01689; X64592; X65735; J01688 

Genbank Nucleotide ID

 AAA58138.1; AAC76366.1; BAE77950.1; CAA23649.1; AAA50990.1; CAA45881.1; CAA46644.1; AAA50989.1 

Protein Name

 30S ribosomal protein S7 

Protein Synonyms/Alias

  

Gene Name

 rpsG 

Gene Synonyms/Alias

 b3341; JW3303 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
17	RKILPDPKFGSELLA	acetylation	[1]
56	TLAQRSGKSELEAFE	acetylation	[1]
56	TLAQRSGKSELEAFE	pupylation	[2]
76	VRPTVEVKSRRVGGS	acetylation	[1]
114	AARKRGDKSMALRLA	acetylation	[1]
131	LSDAAENKGTAVKKR	acetylation	[1]
131	LSDAAENKGTAVKKR	pupylation	[2]
149	HRMAEANKAFAHYRW	acetylation	[1, 3]
171	HQAGASSKQPALGYL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding; tRNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 179 AA 

Protein Sequence

Gene Ontology

 GO:0022627; C:cytosolic small ribosomal subunit; IDA:EcoCyc.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0003729; F:mRNA binding; IDA:EcoCyc.
 GO:0019843; F:rRNA binding; IDA:EcoCyc.
 GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
 GO:0000049; F:tRNA binding; IEA:HAMAP.
 GO:0017148; P:negative regulation of translation; IDA:EcoCyc.
 GO:0000028; P:ribosomal small subunit assembly; IDA:EcoCyc.
 GO:0006412; P:translation; IEA:HAMAP. 

Interpro

 IPR000235; Ribosomal_S5/S7.
 IPR005717; Ribosomal_S7_bac/org-type.
 IPR020606; Ribosomal_S7_CS.
 IPR023798; Ribosomal_S7_dom. 

Pfam

 PF00177; Ribosomal_S7 

SMART

  

PROSITE

 PS00052; RIBOSOMAL_S7 

PRINTS