UniProt Accession

 NIT3_YEAST; P49954; D6VYY9; Q71SP9 

Genbank Protein ID

 AF284572; U19102; BK006945 

Genbank Nucleotide ID

 AAF87101.1; AAB67751.1; DAA09655.1 

Protein Name

 Probable hydrolase NIT3 

Protein Synonyms/Alias

  

Gene Name

 NIT3 

Gene Synonyms/Alias

 YLR351C; L9638.5 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
47	MKEQPDTKLVVLPEC	ubiquitination	[1]
105	TIPELDPKTDKIYNT	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

  

Sequence Annotation

 DOMAIN 11 286 CN hydrolase.
 ACT_SITE 53 53 Proton acceptor (Potential).
 ACT_SITE 128 128 Probable.
 ACT_SITE 169 169 Nucleophile (Probable).
 MOD_RES 34 34 Phosphothreonine.  

Keyword

 3D-structure; Complete proteome; Hydrolase; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 291 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0016810; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; ISS:SGD.
 GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro. 

Interpro

 IPR003010; C-N_Hydrolase.
 IPR001110; UPF0012_CS. 

Pfam

 PF00795; CN_hydrolase 

SMART

  

PROSITE

 PS50263; CN_HYDROLASE
 PS01227; UPF0012 

PRINTS