CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044145
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Diphthamide biosynthesis protein 1 
Protein Synonyms/Alias
  
Gene Name
 DPH1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
42QIPPEILKNPQLQAAubiquitination[1, 2, 3]
63NYNFEIPKTIWRIQQubiquitination[3, 4, 5]
197RVSVPQCKPLSPGEIubiquitination[2, 3, 5]
214CTSPRLSKEVEAVVYubiquitination[1]
249YRYDPYSKVLSREHYubiquitination[1, 2, 3, 4, 5]
276IATARSAKSWGLILGubiquitination[1, 2, 3, 4]
402PGRPARGKVQEGSARubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 431 AA 
Protein Sequence
XRQVMAALVV SGAAEQGGRD GPGRGRAPRG RVANQIPPEI LKNPQLQAAI RVLPSNYNFE 60
IPKTIWRIQQ AQAKKVALQM PEGLLLFACT IVDILERFTE AEVMVMGDVT YGACCVDDFT 120
ARALGADFLV HYGHSCLIPM DTSAQDFRVL YVFVDIRIDT THLLDSLRLT FPPATALALA 180
AAQELKAEYR VSVPQCKPLS PGEILGCTSP RLSKEVEAVV YLGDGRFHLE SVMIANPNVP 240
AYRYDPYSKV LSREHYDHQR MQAARQEAIA TARSAKSWGL ILGTLGRQGS PKILEHLESR 300
LRALGLSFVR LLLSEIFPSK LSLLPEVDVW VQVACPRLSI DWGTAFPKPL LTPYEAAVAL 360
RDISWQQPYP MDFYAGSSLG PWTVNHGQDR RPHAPGRPAR GKVQEGSARP PSAVACEDCS 420
CRDEKVAPLA P 431 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; IEA:InterPro. 
Interpro
 IPR002728; DPH1/DPH2.
 IPR016435; eu_DPH1/arc_DPH2. 
Pfam
 PF01866; Diphthamide_syn 
SMART
  
PROSITE
  
PRINTS