CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016485
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Choline dehydrogenase, mitochondrial 
Protein Synonyms/Alias
 CDH; CHD 
Gene Name
 Chdh 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
40RAVASVGKDEYTFVVacetylation[1]
281AVGVEYIKDGQRHKAacetylation[2, 3]
287IKDGQRHKAYVSREVacetylation[2, 3]
438ATSVGWLKLRSANPRacetylation[1, 2, 3, 4]
438ATSVGWLKLRSANPRsuccinylation[4]
486ALAPFRGKELQPGSHacetylation[1, 2, 3, 4]
486ALAPFRGKELQPGSHsuccinylation[4]
498GSHVQSDKEIDAFVRacetylation[1, 2, 3, 4, 5, 6]
498GSHVQSDKEIDAFVRsuccinylation[4]
507IDAFVRAKADSAYHPacetylation[4]
507IDAFVRAKADSAYHPsuccinylation[4]
535AVVDAQTKVIGVENLacetylation[3]
574EKAADIIKGHPALEDacetylation[2]
582GHPALEDKNVPVYKPacetylation[1, 2, 3]
588DKNVPVYKPQTLDTQacetylation[1, 2, 3]
Reference
 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [6] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599
Functional Description
  
Sequence Annotation
 NP_BIND 44 73 FAD (By similarity).
 ACT_SITE 513 513 By similarity.
 MOD_RES 498 498 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 596 AA 
Protein Sequence
MWQVLRGWRK GWQSPRGALA WAVQGQPCPP CSRAVASVGK DEYTFVVVGA GSAGCVLASR 60
LTEDPNHRVL LLEAGPKDLL MGSKRLQWKI HMPAALVSNL CDDKYNWYYH TEPQPGMDSR 120
VLYWPRGRVW GGSSSLNAMV YIRGHAEDYN RWHREGAEGW DYAHCLPYFR KAQRHELGAN 180
MYRGGDGPLH VSRGKTNHPL HQAFLQAARQ AGYPFTEDMN GFQQEGFGWM DMTVHQGKRW 240
STACAYLHPV LSRPNLRAEV QTLVSRVLFE GTRAVGVEYI KDGQRHKAYV SREVILSGGA 300
INSPQLLMLS GVGNADDLRK LDIPVVCHLP GVGQNLQDHL EVYVQQACTQ PITLHSAQKP 360
LRKVCIGLEW LWSYTGDGAT AHLETGGFIR SRPGVPHPDI QFHFLPSQVI DHGRKPTQQE 420
AYQVHVGTMR ATSVGWLKLR SANPRDHPVI HPNYLSTETD VEDFRQCVRL SREIFAQEAL 480
APFRGKELQP GSHVQSDKEI DAFVRAKADS AYHPSCTCKM GRSSDPTAVV DAQTKVIGVE 540
NLRVVDASIM PSVVSGNLNA PTVMIAEKAA DIIKGHPALE DKNVPVYKPQ TLDTQR 596 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0008812; F:choline dehydrogenase activity; IEA:EC.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0019285; P:glycine betaine biosynthetic process from choline; IEA:UniProtKB-UniPathway. 
Interpro
 IPR011533; Choline_dehydrogenase.
 IPR012132; GMC_OxRdtase.
 IPR000172; GMC_OxRdtase_N.
 IPR007867; GMC_OxRtase_C. 
Pfam
 PF05199; GMC_oxred_C
 PF00732; GMC_oxred_N 
SMART
  
PROSITE
 PS00623; GMC_OXRED_1
 PS00624; GMC_OXRED_2 
PRINTS