CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030619
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histidine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Histidyl-tRNA synthetase, isoform CRA_a; cDNA FLJ42841 fis, clone BRCOC2003213, highly similar to Histidyl-tRNA synthetase (EC 6.1.1.21) 
Gene Name
 HARS 
Gene Synonyms/Alias
 hCG_1782886 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
6**MAVREKVFDVIIRubiquitination[1, 2, 3]
37LKETLMGKYGEDSKLubiquitination[2, 4]
43GKYGEDSKLIYDLKDacetylation[3]
43GKYGEDSKLIYDLKDubiquitination[2, 3, 4]
49SKLIYDLKDQGGELLubiquitination[2, 3, 4, 5]
74ARYLAMNKLTNIKRYubiquitination[2]
161ICGVSDSKFRTICSSubiquitination[2, 3, 5]
181KVSWEEVKNEMVGEKubiquitination[2, 3, 4, 5]
188KNEMVGEKGLAPEVAubiquitination[2, 3, 4, 5, 6]
219EQLLQDPKLSQNKQAubiquitination[2, 4]
224DPKLSQNKQALEGLGubiquitination[1, 3, 4, 5, 6]
304LVGMFDPKGRKVPCVubiquitination[2, 3, 4]
307MFDPKGRKVPCVGLSubiquitination[2]
334RLEALEEKIRTTETQubiquitination[2, 3, 4, 6]
349VLVASAQKKLLEERLubiquitination[2, 4]
357KLLEERLKLVSELWDubiquitination[2]
430EDLVEEIKRRTGQPLubiquitination[2, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Aminoacyl-tRNA synthetase; Complete proteome; Ligase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 440 AA 
Protein Sequence
MAVREKVFDV IIRCFKRHGA EVIDTPVFEL KETLMGKYGE DSKLIYDLKD QGGELLSLRY 60
DLTVPFARYL AMNKLTNIKR YHIAKVYRRD NPAMTRGRYR EFYQCDFDIA GNFDPMIPDA 120
ECLKIMCEIL SSLQIGDFLV KVNDRRILDG MFAICGVSDS KFRTICSSVD KLDKVSWEEV 180
KNEMVGEKGL APEVADRIGD YVQQHGGVSL VEQLLQDPKL SQNKQALEGL GDLKLLFEYL 240
TLFGIDDKIS FDLSLARGLD YYTGVIYEAV LLQTPAQAGE EPLGVGSVAA GGRYDGLVGM 300
FDPKGRKVPC VGLSIGVERI FSIVEQRLEA LEEKIRTTET QVLVASAQKK LLEERLKLVS 360
ELWDAGIKAE LLYKKNPKLL NQLQYCEEAG IPLVAIIGEQ ELKDGVIKLR SVTSREEVDV 420
RREDLVEEIK RRTGQPLCIC 440 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004821; F:histidine-tRNA ligase activity; IEA:InterPro.
 GO:0006427; P:histidyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR015807; His-tRNA-ligase.
 IPR004516; HisRS/HisZ. 
Pfam
 PF03129; HGTP_anticodon 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS