CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004614
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CTP synthase 1 
Protein Synonyms/Alias
 CTP synthetase 1; UTP--ammonia ligase 1 
Gene Name
 CTPS1 
Gene Synonyms/Alias
 CTPS 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
84FLDIRLTKDNNLTTGubiquitination[1]
92DNNLTTGKIYQYVINubiquitination[1, 2, 3, 4]
100IYQYVINKERKGDYLacetylation[5]
100IYQYVINKERKGDYLubiquitination[1, 2, 3, 4, 6, 7]
169AFRQFQFKVKRENFCubiquitination[1]
193PSSTGEQKTKPTQNSubiquitination[1]
195STGEQKTKPTQNSVRubiquitination[1]
227NPLDTSVKEKISMFCubiquitination[1]
229LDTSVKEKISMFCHVubiquitination[1]
286RKMLMKWKEMADRYDubiquitination[1]
306CSIALVGKYTKFSDSubiquitination[1]
309ALVGKYTKFSDSYASubiquitination[1, 2, 3, 4, 8]
319DSYASVIKALEHSALubiquitination[1, 2, 3, 4, 8]
331SALAINHKLEIKYIDubiquitination[1]
335INHKLEIKYIDSADLubiquitination[1, 3]
360RYHEAWQKLCSAHGVubiquitination[1]
381GVRGTEGKIQAIAWAubiquitination[1]
460RRTLFQTKNSVMRKLubiquitination[1, 2, 4]
466TKNSVMRKLYGDADYubiquitination[1]
490EVNPVWKKCLEEQGLubiquitination[1]
498CLEEQGLKFVGQDVEubiquitination[1]
535EFLSRPIKPSPPYFGubiquitination[1, 2, 4]
557RLSHYLQKGCRLSPRubiquitination[1, 8, 9]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. 
Sequence Annotation
 DOMAIN 300 554 Glutamine amidotransferase type-1.
 ACT_SITE 399 399 For GATase activity (By similarity).
 ACT_SITE 526 526 For GATase activity (By similarity).
 ACT_SITE 528 528 For GATase activity (By similarity).
 MOD_RES 100 100 N6-acetyllysine.
 MOD_RES 562 562 Phosphoserine.
 MOD_RES 566 566 Phosphothreonine (By similarity).
 MOD_RES 567 567 Phosphotyrosine (By similarity).
 MOD_RES 568 568 Phosphoserine.
 MOD_RES 571 571 Phosphoserine.
 MOD_RES 573 573 Phosphoserine.
 MOD_RES 574 574 Phosphoserine.
 MOD_RES 575 575 Phosphoserine.
 MOD_RES 587 587 Phosphoserine.  
Keyword
 3D-structure; Acetylation; ATP-binding; Complete proteome; Glutamine amidotransferase; Ligase; Nucleotide-binding; Phosphoprotein; Polymorphism; Pyrimidine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 591 AA 
Protein Sequence
MKYILVTGGV ISGIGKGIIA SSVGTILKSC GLHVTSIKID PYINIDAGTF SPYEHGEVFV 60
LDDGGEVDLD LGNYERFLDI RLTKDNNLTT GKIYQYVINK ERKGDYLGKT VQVVPHITDA 120
IQEWVMRQAL IPVDEDGLEP QVCVIELGGT VGDIESMPFI EAFRQFQFKV KRENFCNIHV 180
SLVPQPSSTG EQKTKPTQNS VRELRGLGLS PDLVVCRCSN PLDTSVKEKI SMFCHVEPEQ 240
VICVHDVSSI YRVPLLLEEQ GVVDYFLRRL DLPIERQPRK MLMKWKEMAD RYDRLLETCS 300
IALVGKYTKF SDSYASVIKA LEHSALAINH KLEIKYIDSA DLEPITSQEE PVRYHEAWQK 360
LCSAHGVLVP GGFGVRGTEG KIQAIAWARN QKKPFLGVCL GMQLAVVEFS RNVLGWQDAN 420
STEFDPTTSH PVVVDMPEHN PGQMGGTMRL GKRRTLFQTK NSVMRKLYGD ADYLEERHRH 480
RFEVNPVWKK CLEEQGLKFV GQDVEGERME IVELEDHPFF VGVQYHPEFL SRPIKPSPPY 540
FGLLLASVGR LSHYLQKGCR LSPRDTYSDR SGSSSPDSEI TELKFPSINH D 591 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003883; F:CTP synthase activity; IDA:UniProtKB.
 GO:0044210; P:'de novo' CTP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006241; P:CTP biosynthetic process; IDA:UniProtKB.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0015949; P:nucleobase-containing small molecule interconversion; TAS:Reactome.
 GO:0042493; P:response to drug; TAS:ProtInc. 
Interpro
 IPR004468; CTP_synthase.
 IPR017456; CTP_synthase_N.
 IPR017926; GATASE.
 IPR027417; P-loop_NTPase. 
Pfam
 PF06418; CTP_synth_N
 PF00117; GATase 
SMART
  
PROSITE
 PS51273; GATASE_TYPE_1 
PRINTS