CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023801
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Small kinetochore-associated protein 
Protein Synonyms/Alias
 SKAP; Kinetochore-localized astrin-binding protein; Kinastrin; Kinetochore-localized astrin/SPAG5-binding protein; TRAF4-associated factor 1 
Gene Name
 KNSTRN 
Gene Synonyms/Alias
 C15orf23; SKAP; TRAF4AF1; HSD11 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
212QKVELLEKFRDNCLAubiquitination[1, 2]
224CLAILESKGLDPALGubiquitination[3, 4]
268LFNETAKKQMEELQAmethylation[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510] 
Functional Description
 Essential component of the mitotic spindle required for faithful chromosome segregation and progression into anaphase. Promotes the metaphase-to-anaphase transition and is required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments. 
Sequence Annotation
 REGION 159 316 Interaction with SPAG5.
 MOD_RES 128 128 Phosphoserine.  
Keyword
 Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 316 AA 
Protein Sequence
MAAPEAPPLD RVFRTTWLST ECDSHPLPPS YRKFLFETQA ADLAGGTTVA AGNLLNESEK 60
DCGQDRRAPG VQPCRLVTMT SVVKTVYSLQ PPSALSGGQP ADTQTRATSK SLLPVRSKEV 120
DVSKQLHSGG PENDVTKITK LRRENGQMKA TDTATRRNVR KGYKPLSKQK SEEELKDKNQ 180
LLEAVNKQLH QKLTETQGEL KDLTQKVELL EKFRDNCLAI LESKGLDPAL GSETLASRQE 240
STTDHMDSML LLETLQEELK LFNETAKKQM EELQALKVKL EMKEERVRFL EQQTLCNNQV 300
NDLTTALKEM EQLLEM 316 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0035371; C:microtubule plus end; IDA:UniProtKB.
 GO:0072686; C:mitotic spindle; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000090; P:mitotic anaphase; IMP:UniProtKB.
 GO:0000070; P:mitotic sister chromatid segregation; IMP:UniProtKB.
 GO:0051988; P:regulation of attachment of spindle microtubules to kinetochore; IMP:UniProtKB.
 GO:0007051; P:spindle organization; IMP:UniProtKB. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS