CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024548
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Clathrin interactor 1 
Protein Synonyms/Alias
 Epsin-4 
Gene Name
 CLINT1 
Gene Synonyms/Alias
 EPN4 
Created Date
 July 27, 2013 
Organism
 Bos taurus (Bovine) 
NCBI Taxa ID
 9913 
Lysine Modification
Position
Peptide
Type
References
183YDPEPKSKWDEEWDKubiquitination[1]
190KWDEEWDKNKSAFPFubiquitination[1]
192DEEWDKNKSAFPFSDubiquitination[1]
200SAFPFSDKLGELSDKubiquitination[2]
207KLGELSDKIGSTIDDubiquitination[1]
218TIDDTISKFRRKDREubiquitination[1]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly (By similarity). 
Sequence Annotation
 DOMAIN 16 149 ENTH.
 BINDING 29 29 Phosphatidylinositol lipid headgroup (By
 BINDING 67 67 Phosphatidylinositol lipid headgroup (By
 MOD_RES 163 163 Phosphoserine (By similarity).
 MOD_RES 227 227 Phosphoserine (By similarity).
 MOD_RES 232 232 Phosphoserine (By similarity).
 MOD_RES 234 234 Phosphoserine (By similarity).
 MOD_RES 245 245 Phosphoserine (By similarity).
 MOD_RES 299 299 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Cytoplasmic vesicle; Endocytosis; Lipid-binding; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 643 AA 
Protein Sequence
MLNMWKVREL VDKATNVVMN YSEIESKVRE ATNDDPWGPS GQLMGEIAKA TFMYEQFPEL 60
MNMLWSRMLK DNKKNWRRVY KSLLLLAYLI RNGSERVVTS AREHIYDLRS LENYHFVDEH 120
GKDQGINIRQ KVKELVEFAQ DDDRLREERK KAKKNKDKYV GVSSDSVGGF RYSERYDPEP 180
KSKWDEEWDK NKSAFPFSDK LGELSDKIGS TIDDTISKFR RKDREDSPER CSDSDEEKKA 240
RRGRSPKGEF KDEEETVTTK HIHITQATET TTTRHKRTAN PSKTIDLGAA AHYTGDKASP 300
DQNASTHTPQ SSLKTSVPSS KSSGDLVDLF DGTSQSTGGS ADLFGGFADF GSAAASGNFP 360
SQVTATSGNG DFGDWSAFNQ APSVPVAASG ELFGSASQPA VELVSSSQPA LGPPPAASNS 420
SDLFDLMGSS QATMTSSQSM NFSMMSTNTV GLGLPMSRSQ PLQNVSTVLQ KPNPLYNQNT 480
DMVQKSVSKT LPSTWSDPSV NISLDNLLPG MQPSKPQQPS LNTMIQQQNM QQPMNMMTQS 540
FGAVNLSSPS NMLPVRPQTN PLMGGPMPMS MPNVMTGTMG MAPLGNSPMM NQSMMGMNMN 600
IGMSTTGMGL TGTMGMGMPN LAMTSGTMQP KQDAFANFAN FSK 643 
Gene Ontology
 GO:0030136; C:clathrin-coated vesicle; IEA:UniProtKB-SubCell.
 GO:0005798; C:Golgi-associated vesicle; TAS:BHF-UCL.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0005802; C:trans-Golgi network; TAS:BHF-UCL.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW. 
Interpro
 IPR008942; ENTH_VHS.
 IPR013809; Epsin-like_N.
 IPR001026; Epsin_dom_N. 
Pfam
 PF01417; ENTH 
SMART
 SM00273; ENTH 
PROSITE
 PS50942; ENTH 
PRINTS