CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032052
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 
Protein Synonyms/Alias
 cDNA FLJ58271, highly similar to 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 
Gene Name
 PFKFB4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
93EEGLKIRKQCALAALubiquitination[1]
254GESELNLKGRIGGDPubiquitination[1]
284FISDQNIKDLKVWTSubiquitination[1]
294KVWTSQMKRTIQTAEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Kinase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 458 AA 
Protein Sequence
MDRGSLGGSS LTDLGPGSSR RVCMTNCPTL IVMVGLPARG KTYISKKLTR YLNWIGVPTR 60
EFNVGQYRRD VVKTYKSFEF FLPDNEEGLK IRKQCALAAL RDVRRFLSEE GGHVAVFDAT 120
NTTRERRATI FNFGEQNGYK TFFVESICVD PEVIAANIVQ VKLGSPDYVN RDSDEATEDF 180
MRRIECYENS YESLDEDLDR DLSYIKIMDV GQSYVVNRVA DHIQSRIVYY LMNIHVTPRS 240
IYLCRHGESE LNLKGRIGGD PGLSPRGREF AKSLAQFISD QNIKDLKVWT SQMKRTIQTA 300
EALGVPYEQW KVLNEIDAGV CEEMTYEEIQ DNYPLEFALR DQDKYRYRYP KGESYEDLVQ 360
RLEPVIMELE RQENVLVICH QAVMRCLLAY FLDKAAEQLP YLKCPLHTVL KLTPVAYGCK 420
VESIFLNVAA VNTHRDRPQN VDISRPPEEA LVTVPAHQ 458 
Gene Ontology
 GO:0003873; F:6-phosphofructo-2-kinase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004331; F:fructose-2,6-bisphosphate 2-phosphatase activity; IEA:InterPro.
 GO:0006003; P:fructose 2,6-bisphosphate metabolic process; IEA:InterPro.
 GO:0006000; P:fructose metabolic process; IEA:InterPro. 
Interpro
 IPR003094; 6Pfruct_kin.
 IPR013079; 6Phosfructo_kin.
 IPR016260; Bifunct_6PFK/fruc_bisP_Ptase.
 IPR013078; His_Pase_superF_clade-1.
 IPR027417; P-loop_NTPase.
 IPR001345; PG/BPGM_mutase_AS. 
Pfam
 PF01591; 6PF2K
 PF00300; His_Phos_1 
SMART
 SM00855; PGAM 
PROSITE
 PS00175; PG_MUTASE 
PRINTS
 PR00991; 6PFRUCTKNASE.