UniProt Accession

 RFA2_HUMAN; P15927; Q52II0; Q5TEI9; Q5TEJ5 

Genbank Protein ID

 J05249; CR450348; DQ001128; AL109927; AL109927; AL109927; BC001630; BC012157; BC021257 

Genbank Nucleotide ID

 AAA36560.1; CAG29344.1; AAX84514.1; CAI21777.1; CAI21778.1; CAI21775.1; AAH01630.1; AAH12157.1; AAH21257.1 

Protein Name

 Replication protein A 32 kDa subunit 

Protein Synonyms/Alias

 RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34 

Gene Name

 RPA2 

Gene Synonyms/Alias

 REPA2; RPA32; RPA34 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
173	GIIRHAEKAPTNIVY	ubiquitination	[1]
181	APTNIVYKIDDMTAA	ubiquitination	[1]
215	VPPETYVKVAGHLRS	ubiquitination	[1, 2, 3]
323	QDLKNQLKHMSVSSI	ubiquitination	[4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. 

Sequence Annotation

 REGION 187 270 Interaction with TIPIN (By similarity).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 21 21 Phosphothreonine; by PRKDC; in vitro.
 MOD_RES 23 23 Phosphoserine.
 MOD_RES 29 29 Phosphoserine; by CDK1; in vitro.
 MOD_RES 33 33 Phosphoserine; by PRKDC; in vitro.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; DNA damage; DNA recombination; DNA repair; DNA replication; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 270 AA 

Protein Sequence

Gene Ontology

 GO:0005662; C:DNA replication factor A complex; IPI:MGI.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0003697; F:single-stranded DNA binding; TAS:ProtInc.
 GO:0000730; P:DNA recombinase assembly; TAS:Reactome.
 GO:0006271; P:DNA strand elongation involved in DNA replication; TAS:Reactome.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome.
 GO:0000718; P:nucleotide-excision repair, DNA damage removal; TAS:Reactome.
 GO:0006297; P:nucleotide-excision repair, DNA gap filling; TAS:Reactome.
 GO:0010569; P:regulation of double-strand break repair via homologous recombination; IMP:UniProtKB.
 GO:0000722; P:telomere maintenance via recombination; TAS:Reactome.
 GO:0032201; P:telomere maintenance via semi-conservative replication; TAS:Reactome.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome. 

Interpro

 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase.
 IPR014646; RPA32.
 IPR014892; RPA_C.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF08784; RPA_C
 PF01336; tRNA_anti 

SMART

  

PROSITE

  

PRINTS