CPLM-022986

Genbank Nucleotide ID

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
653	QLEKTELKMDFLRER	ubiquitination	[1]
668	ELRETLEKQLAMEQK	ubiquitination	[1]
713	EQAEQTLKQAASTDS	ubiquitination	[2]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin- dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain (By similarity).

REGION 191 386 Interaction with SIX6 and HDAC3 (By
REGION 191 277 DACHbox-N.
REGION 618 698 DACHbox-C.
REGION 629 708 Interaction with SIN3A (By similarity).

3D-structure; Activator; Alternative splicing; Coiled coil; Complete proteome; Developmental protein; DNA-binding; Nucleus; Reference proteome; Repressor; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:Compara.
GO:0005730; C:nucleolus; IDA:HPA.
GO:0005667; C:transcription factor complex; IEA:Compara.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0001078; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; IDA:BHF-UCL.
GO:0001075; F:RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly; IEA:Compara.
GO:0008283; P:cell proliferation; IEA:Compara.
GO:0046545; P:development of primary female sexual characteristics; IEA:Compara.
GO:0030336; P:negative regulation of cell migration; IDA:BHF-UCL.
GO:0060244; P:negative regulation of cell proliferation involved in contact inhibition; IEA:Compara.
GO:0048147; P:negative regulation of fibroblast proliferation; IEA:Compara.
GO:0010944; P:negative regulation of transcription by competitive promoter binding; IMP:BHF-UCL.
GO:0007585; P:respiratory gaseous exchange; IEA:Compara.
GO:0001967; P:suckling behavior; IEA:Compara.

IPR009061; DNA-bd_dom_put.
IPR003380; Transform_Ski.