CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036071
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 E3 ubiquitin-protein ligase MIB2 
Protein Synonyms/Alias
  
Gene Name
 MIB2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
176RGIFQGAKVVRGPDWubiquitination[1]
194SQDGGEGKPGRVVDIubiquitination[2]
276QPFQHGDKVKCLLDTubiquitination[1]
278FQHGDKVKCLLDTDVubiquitination[1, 3, 4]
358IGDLDTVKRLQAGHGubiquitination[1, 2]
383GRVGKVVKVFGDGNLubiquitination[1, 3]
436SLSVALDKLRAQKSDubiquitination[1]
645RQLVDAKKEDGFTALubiquitination[1]
798AAEGRVLKALQGCAQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 ANK repeat; Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 816 AA 
Protein Sequence
MDPSAHRSRA APPNMDPDPQ AGVQVGMRVV RGVDWKWGQQ DGGEGGVGTV VELGRHGSPS 60
TPDRTVVVQW DQGTRTNYRA GYQGAHDLLL YDNAQIGVRH PNIICDCCKK HGLRGMRWKC 120
RVCLDYDLCT QCYMHNKHEL AHAFDRYETA HSRPVTLSPR QGLPRIPLRG IFQGAKVVRG 180
PDWEWGSQDG GEGKPGRVVD IRGWDVETGR SVASVTWADG TTNVYRVGHK GKVDLKCVGE 240
AAGGFYYKDH LPRLGKPAEL QRRVSADSQP FQHGDKVKCL LDTDVLREMQ EGHGGWNPRM 300
AEFIGQTGTV HRITDRGDVR VQFNHETRWT FHPGALTKHH SFWVGDVVRV IGDLDTVKRL 360
QAGHGEWTDD MAPALGRVGK VVKVFGDGNL RVAVAGQRWT FSPSCLVAYR PEEDANLDVA 420
ERARENKSSL SVALDKLRAQ KSDPEHPGRL VVEVALGNAA RALDLLRRRP EQVDTKNQGR 480
TALQVAAYLG QVELIRLLLQ ARAGVDLPDD EGNTALHYAA LGNQPEATRV LLSAGCRADA 540
INSTQSTALH VAVQRGFLEV VRALCERGCD VNLPDAHSDT PLHSAISAGT GASGIVEVLT 600
EVPNIDVTAT NSQGFTLLHH ASLKGHALAV RKILARARQL VDAKKEDGFT ALHLAALNNH 660
REVAQILIRE GRCDVNVRNR KLQSPLHLAV QQAHVGLVPL LVDAGCSVNA EDEEGDTALH 720
VALQRHQLLP LVADGAGGDP GPLQLLSRLQ ASGLPGSAEL TVGAAVACFL ALEGADVSYT 780
NHRGRSPLDL AAEGRVLKAL QGCAQRFRER QAGGGR 816 
Gene Ontology
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR010606; Mib_Herc2.
 IPR000433; Znf_ZZ. 
Pfam
 PF12796; Ank_2
 PF06701; MIB_HERC2
 PF00569; ZZ 
SMART
 SM00248; ANK
 SM00291; ZnF_ZZ 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS51416; MIB_HERC2
 PS01357; ZF_ZZ_1
 PS50135; ZF_ZZ_2 
PRINTS
 PR01415; ANKYRIN.