UniProt Accession

 PRP4_MOUSE; Q9DAW6 

Genbank Protein ID

 AK005464; AK089838; AK166912; AK167216; AK169532; BC106089 

Genbank Nucleotide ID

 BAB24055.1; BAC40976.1; BAE39112.1; BAE39342.1; BAE41214.1; AAI06090.1 

Protein Name

 U4/U6 small nuclear ribonucleoprotein Prp4 

Protein Synonyms/Alias

 U4/U6 snRNP 60 kDa protein; WD splicing factor Prp4 

Gene Name

 Prpf4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
26	LVAPVVKKPHIYYGS	acetylation	[1, 2]
145	VVGTDALKKTKKDDE	ubiquitination	[3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (By similarity). 

Sequence Annotation

 REPEAT 228 267 WD 1.
 REPEAT 270 317 WD 2.
 REPEAT 320 359 WD 3.
 REPEAT 362 401 WD 4.
 REPEAT 404 443 WD 5.
 REPEAT 446 486 WD 6.
 REPEAT 489 521 WD 7.
 MOD_RES 26 26 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; Spliceosome; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 521 AA 

Protein Sequence

Gene Ontology

 GO:0015030; C:Cajal body; IEA:Compara.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
 GO:0071001; C:U4/U6 snRNP; IEA:Compara.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 

Interpro

 IPR020472; G-protein_beta_WD-40_rep.
 IPR014906; PRP4.
 IPR027106; Prp4/Asa1.
 IPR003648; SFM.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF08799; PRP4
 PF00400; WD40 

SMART

 SM00500; SFM
 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS

 PR00320; GPROTEINBRPT.