| Tag | Content |
|---|
| CPLM ID | CPLM-011455 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Elongator complex protein 1 |
Protein Synonyms/Alias | Gamma-toxin target 1; Protein IKI3 |
Gene Name | IKI3 |
Gene Synonyms/Alias | ELP1; TOT1; YLR384C; L3502.7 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 967 | DNEPLRRKFLIDDYL | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. Independently, ELP3 may be involved in polarized exocytosis. It is required for the polarized localization of GTPase-activating protein SEC2. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA. |
Sequence Annotation | |
Keyword | Complete proteome; Cytoplasm; Nucleus; Protein transport; Reference proteome; Transcription; Transcription regulation; Transport. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1349 AA |
Protein Sequence | MVEHDKSGSK RQELRSNMRN LITLNKGKFK PTASTAEGDE DDLSFTLLDS VFDTLSDSIT 60
CVLGSTDIGA IEVQQFMKDG SRNVLASFNI QTFDDKLLSF VHFADINQLV FVFEQGDIIT 120
ATYDPVSLDP AETLIEIMGT IDNGIAAAQW SYDEETLAMV TKDRNVVVLS KLFEPISEYH 180
LEVDDLKISK HVTVGWGKKE TQFRGKGARA MEREALASLK ASGLVGNQLR DPTMPYMVDT 240
GDVTALDSHE ITISWRGDCD YFAVSSVEEV PDEDDETKSI KRRAFRVFSR EGQLDSASEP 300
VTGMEHQLSW KPQGSLIASI QRKTDLGEED SVDVIFFERN GLRHGEFDTR LPLDEKVESV 360
CWNSNSEALA VVLANRIQLW TSKNYHWYLK QELYASDISY VKWHPEKDFT LMFSDAGFIN 420
IVDFAYKMAQ GPTLEPFDNG TSLVVDGRTV NITPLALANV PPPMYYRDFE TPGNVLDVAC 480
SFSNEIYAAI NKDVLIFAAV PSIEEMKKGK HPSIVCEFPK SEFTSEVDSL RQVAFINDSI 540
VGVLLDTDNL SRIALLDIQD ITQPTLITIV EVYDKIVLLR SDFDYNHLVY ETRDGTVCQL 600
DAEGQLMEIT KFPQLVRDFR VKRVHNTSAE DDDNWSAESS ELVAFGITNN GKLFANQVLL 660
ASAVTSLEIT DSFLLFTTAQ HNLQFVHLNS TDFKPLPLVE EGVEDERVRA IERGSILVSV 720
IPSKSSVVLQ ATRGNLETIY PRIMVLAEVR KNIMAKRYKE AFIVCRTHRI NLDILHDYAP 780
ELFIENLEVF INQIGRVDYL NLFISCLSED DVTKTKYKET LYSGISKSFG MEPAPLTEMQ 840
IYMKKKMFDP KTSKVNKICD AVLNVLLSNP EYKKKYLQTI ITAYASQNPQ NLSAALKLIS 900
ELENSEEKDS CVTYLCFLQD VNVVYKSALS LYDVSLALLV AQKSQMDPRE YLPFLQELQD 960
NEPLRRKFLI DDYLGNYEKA LEHLSEIDKD GNVSEEVIDY VESHDLYKHG LALYRYDSEK 1020
QNVIYNIYAK HLSSNQMYTD AAVAYEMLGK LKEAMGAYQS AKRWREAMSI AVQKFPEEVE 1080
SVAEELISSL TFEHRYVDAA DIQLEYLDNV KEAVALYCKA YRYDIASLVA IKAKKDELLE 1140
EVVDPGLGEG FGIIAELLAD CKGQINSQLR RLRELRAKKE ENPYAFYGQE TEQADDVSVA 1200
PSETSTQESF FTRYTGKTGG TAKTGASRRT AKNKRREERK RARGKKGTIY EEEYLVQSVG 1260
RLIERLNQTK PDAVRVVEGL CRRNMREQAH QIQKNFVEVL DLLKANVKEI YSISEKDRER 1320
VNENGEVYYI PEIPVPEIHD FPKSHIVDF 1349 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:SGD. GO:0033588; C:Elongator holoenzyme complex; IDA:SGD. GO:0005634; C:nucleus; IDA:SGD. GO:0015031; P:protein transport; IEA:UniProtKB-KW. GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IMP:SGD. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. GO:0002098; P:tRNA wobble uridine modification; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |