CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023922
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fatty acid desaturase 3 
Protein Synonyms/Alias
 Cytochrome b5-related protein 
Gene Name
 FADS3 
Gene Synonyms/Alias
 CYB5RP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
37AHDQPGDKWLVIERRubiquitination[1, 2, 3]
86QDLNFVRKFLQPLLIubiquitination[1, 3]
225RHFQHHAKPNIFHKDubiquitination[4]
252ESSVEYGKKKRRYLPubiquitination[1]
405SRVAPLVKSLCAKHGubiquitination[2, 4]
433DIVRSLKKSGDIWLDubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
  
Sequence Annotation
 DOMAIN 20 97 Cytochrome b5 heme-binding.
 MOTIF 182 186 Histidine box-1.
 MOTIF 219 223 Histidine box-2.
 MOTIF 383 387 Histidine box-3.  
Keyword
 Complete proteome; Electron transport; Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 445 AA 
Protein Sequence
MGGVGEPGPR EGPAQPGAPL PTFCWEQIRA HDQPGDKWLV IERRVYDISR WAQRHPGGSR 60
LIGHHGAEDA TDAFRAFHQD LNFVRKFLQP LLIGELAPEE PSQDGPLNAQ LVEDFRALHQ 120
AAEDMKLFDA SPTFFAFLLG HILAMEVLAW LLIYLLGPGW VPSALAAFIL AISQAQSWCL 180
QHDLGHASIF KKSWWNHVAQ KFVMGQLKGF SAHWWNFRHF QHHAKPNIFH KDPDVTVAPV 240
FLLGESSVEY GKKKRRYLPY NQQHLYFFLI GPPLLTLVNF EVENLAYMLV CMQWADLLWA 300
ASFYARFFLS YLPFYGVPGV LLFFVAVRVL ESHWFVWITQ MNHIPKEIGH EKHRDWVSSQ 360
LAATCNVEPS LFTNWFSGHL NFQIEHHLFP RMPRHNYSRV APLVKSLCAK HGLSYEVKPF 420
LTALVDIVRS LKKSGDIWLD AYLHQ 445 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0016020; C:membrane; NAS:UniProtKB.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0016717; F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; IEA:InterPro.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0006636; P:unsaturated fatty acid biosynthetic process; NAS:UniProtKB. 
Interpro
 IPR001199; Cyt_B5-like_heme/steroid-bd.
 IPR012171; Fatty_acid/sphinglp_desaturase.
 IPR005804; Fatty_acid_desaturase-1. 
Pfam
 PF00173; Cyt-b5
 PF00487; FA_desaturase 
SMART
  
PROSITE
 PS00191; CYTOCHROME_B5_1
 PS50255; CYTOCHROME_B5_2 
PRINTS