UniProt Accession

 KAT5_MOUSE; Q8CHK4; A1L394; Q8CGZ3; Q8CGZ4; Q8VIH0 

Genbank Protein ID

 AY061983; AF528194; AF528195; AF528196; AB055409; BC129968 

Genbank Nucleotide ID

 AAL34981.1; AAN77140.1; AAN77141.1; AAN77142.1; BAC53807.1; AAI29969.1 

Protein Name

 Histone acetyltransferase KAT5 

Protein Synonyms/Alias

 60 kDa Tat-interactive protein; Tip60; Histone acetyltransferase HTATIP; Lysine acetyltransferase 5 

Gene Name

 Kat5 

Gene Synonyms/Alias

 Htatip; Tip60 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
52	VHYIDFNKRLDEWVT	acetylation	[1]
104	ASAQASGKTLPIPVQ	acetylation	[2, 3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM (By similarity). 

Sequence Annotation

 ZN_FING 261 283 C2HC-type.
 REGION 377 383 Acetyl-CoA binding (By similarity).
 ACT_SITE 327 327 By similarity.
 ACT_SITE 369 369 Nucleophile (By similarity).
 BINDING 372 372 Acetyl-CoA (By similarity).
 BINDING 407 407 Acetyl-CoA (By similarity).
 MOD_RES 52 52 N6-acetyllysine (By similarity).
 MOD_RES 86 86 Phosphoserine (By similarity).
 MOD_RES 90 90 Phosphoserine; by CDK1 (By similarity).
 MOD_RES 327 327 N6-acetyllysine; by autocatalysis (By
 CROSSLNK 430 430 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 451 451 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Activator; Acyltransferase; Alternative splicing; Chromatin regulator; Complete proteome; Cytoplasm; Growth regulation; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Transferase; Ubl conjugation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 513 AA 

Protein Sequence

Gene Ontology

 GO:0000785; C:chromatin; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005730; C:nucleolus; ISS:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0032777; C:Piccolo NuA4 histone acetyltransferase complex; ISS:UniProtKB.
 GO:0005667; C:transcription factor complex; IDA:MGI.
 GO:0004402; F:histone acetyltransferase activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
 GO:0006978; P:DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; ISS:UniProtKB.
 GO:0006302; P:double-strand break repair; IEA:Compara.
 GO:0032703; P:negative regulation of interleukin-2 production; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IGI:MGI.
 GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR016181; Acyl_CoA_acyltransferase.
 IPR000953; Chromo_domain/shadow.
 IPR016197; Chromodomain-like.
 IPR002717; MOZ_SAS.
 IPR025995; Tudor-knot. 

Pfam

 PF01853; MOZ_SAS
 PF11717; Tudor-knot 

SMART

 SM00298; CHROMO 

PROSITE

  

PRINTS