UniProt Accession

 TNNC2_RABIT; P02586 

Genbank Protein ID

 J03462; Y00760 

Genbank Nucleotide ID

 AAA31481.1; CAA68729.1 

Protein Name

 Troponin C, skeletal muscle 

Protein Synonyms/Alias

  

Gene Name

 TNNC2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Oryctolagus cuniculus (Rabbit) 

NCBI Taxa ID

 9986 

Lysine Modification

Position	Peptide	Type	References
21	EEMIAEFKAAFDMFD	acetylation	[1, 2]
38	GGGDISVKELGTVMR	acetylation	[1, 2]
53	MLGQTPTKEELDAII	acetylation	[1, 2]
85	VMMVRQMKEDAKGKS	acetylation	[1, 2]
89	RQMKEDAKGKSEEEL	acetylation	[1, 2]
89	RQMKEDAKGKSEEEL	ubiquitination	[3]
91	MKEDAKGKSEEELAE	acetylation	[1, 2]
91	MKEDAKGKSEEELAE	ubiquitination	[3]
137	EEIESLMKDGDKNND	acetylation	[1, 2]
141	SLMKDGDKNNDGRID	acetylation	[1, 2]
154	IDFDEFLKMMEGVQ*	acetylation	[1, 2]

Reference

 [1] Extensive interactions between troponins C and I. Zero-length cross-linking of troponin I and acetylated troponin C.
 Kobayashi T, Grabarek Z, Gergely J, Collins JH.
 Biochemistry. 1995 Aug 29;34(34):10946-52. [PMID: 7662676]
 [2] Interaction of a troponin I inhibitory peptide with both domains of troponin C.
 Kobayashi T, Leavis PC, Collins JH.
 Biochim Biophys Acta. 1996 May 2;1294(1):25-30. [PMID: 8639710]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. 

Sequence Annotation

 DOMAIN 15 50 EF-hand 1.
 DOMAIN 51 86 EF-hand 2.
 DOMAIN 91 126 EF-hand 3.
 DOMAIN 127 160 EF-hand 4.
 MOD_RES 2 2 N-acetylthreonine.  

Keyword

 3D-structure; Acetylation; Calcium; Complete proteome; Direct protein sequencing; Metal-binding; Muscle protein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 160 AA 

Protein Sequence

Gene Ontology

 GO:0005509; F:calcium ion binding; IEA:InterPro. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom. 

Pfam

 PF13499; EF_hand_5 

SMART

 SM00054; EFh 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS