CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022202
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-box only protein 28 
Protein Synonyms/Alias
  
Gene Name
 FBXO28 
Gene Synonyms/Alias
 KIAA0483 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
108MLNQGFLKVERYHNLubiquitination[1, 2, 3]
118RYHNLCQKQVKAQLPubiquitination[2]
263PSRQEVTKLQQQVKTubiquitination[4]
269TKLQQQVKTNGAGVTubiquitination[1, 3, 4, 5]
294TKVQEQQKQLQDQDQubiquitination[5]
302QLQDQDQKLLEQTQIubiquitination[2]
347QNEESPRKRKKATEAubiquitination[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (By similarity). 
Sequence Annotation
 DOMAIN 61 109 F-box.
 MOD_RES 235 235 Phosphoserine.
 MOD_RES 242 242 Phosphoserine.
 MOD_RES 270 270 Phosphothreonine.
 MOD_RES 344 344 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 368 AA 
Protein Sequence
MAAAAEERMA EEGGGGQGDG GSSLASGSTQ RQPPPPAPQH PQPGSQALPA PALAPDQLPQ 60
NNTLVALPIV AIENILSFMS YDEISQLRLV CKRMDLVCQR MLNQGFLKVE RYHNLCQKQV 120
KAQLPRRESE RRNHSLARHA DILAAVETRL SLLNMTFMKY VDSNLCCFIP GKVIDEIYRV 180
LRYVNSTRAP QRAHEVLQEL RDISSMAMEY FDEKIVPILK RKLPGSDVSG RLMGSPPVPG 240
PSAALTTMQL FSKQNPSRQE VTKLQQQVKT NGAGVTVLRR EISELRTKVQ EQQKQLQDQD 300
QKLLEQTQII GEQNARLAEL ERKLREVMES AVGNSSGSGQ NEESPRKRKK ATEAIDSLRK 360
SKRLRNRK 368 
Gene Ontology
  
Interpro
 IPR001810; F-box_dom_cyclin-like. 
Pfam
 PF00646; F-box 
SMART
  
PROSITE
 PS50181; FBOX 
PRINTS