CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017641
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative ATP-dependent RNA helicase TDRD9 
Protein Synonyms/Alias
 Tudor domain-containing protein 9 
Gene Name
 TDRD9 
Gene Synonyms/Alias
 C14orf75 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
86SEVEYINKYRQLEAQubiquitination[1, 2]
128CIPGTTYKYPDLPISubiquitination[1]
189IVVTQPRKIGASSIAubiquitination[1]
318YIFEVEGKPHSVEEYubiquitination[1]
440SPVPGYRKIILSTNIubiquitination[1]
487LRLSWASKTSCNQRKubiquitination[1]
511YCYRLVHKDFWDNSIubiquitination[1]
537PLGSTILKVKLLDMGubiquitination[1]
539GSTILKVKLLDMGEPubiquitination[1]
570ERTILLLKEVGALAVubiquitination[1]
650HLDGYRNKVNFSGSSubiquitination[1]
658VNFSGSSKSDCIALVubiquitination[1]
669IALVEAFKTWKACRQubiquitination[1]
684TGELRYPKDELNWGRubiquitination[1]
698RLNYIQIKRIREVAEubiquitination[1]
711AELYEELKTRISQFNubiquitination[1]
735MDQEYIYKQRFILQVubiquitination[1]
803FRQCGQVKSIVFDGAubiquitination[1]
811SIVFDGAKAFVEFSRubiquitination[1]
864VQGMNVSKLRNTRVNubiquitination[1]
876RVNVDFQKQTVDPMQubiquitination[1]
1146KLGTPNCKAELHGPFubiquitination[1]
1168HSLTRISKFRCVWIEmethylation[3]
1295ILRAAINKLVCDGPNubiquitination[1]
1305CDGPNGCKCLGPERVubiquitination[1]
1331LGLFCQSKPREKIVPubiquitination[1]
1343IVPKWHEKPYEWNQVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510] 
Functional Description
 Probable ATP-binding RNA helicase which plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with PIWIL4 and the piP-bodies suggests a participation in the secondary piRNAs metabolic process (By similarity). 
Sequence Annotation
 DOMAIN 142 308 Helicase ATP-binding.
 DOMAIN 377 544 Helicase C-terminal.
 DOMAIN 944 1004 Tudor.
 NP_BIND 155 162 ATP (By similarity).
 MOTIF 254 257 DEAH box.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Helicase; Hydrolase; Meiosis; Nucleotide-binding; Nucleus; Reference proteome; RNA-mediated gene silencing; Spermatogenesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1382 AA 
Protein Sequence
MLRKLTIEQI NDWFTIGKTV TNVELLGAPP AFPAGAAREE VQRQDVAPGA GPAAQAPALA 60
QAPARPAAAF ERSLSQRSSE VEYINKYRQL EAQELDVCRS VQPTSGPGPR PSLAKLSSVT 120
CIPGTTYKYP DLPISRYKEE VVSLIESNSV VIIHGATGSG KSTQLPQYIL DHYVQRSAYC 180
SIVVTQPRKI GASSIARWIS KERAWTLGGV VGYQVGLEKI ATEDTRLIYM TTGVLLQKIV 240
SAKSLMEFTH IIIDEVHERT EEMDFLLLVV RKLLRTNSRF VKVVLMSATI SCKEFADYFA 300
VPVQNKMNPA YIFEVEGKPH SVEEYYLNDL EHIHHSKLSP HLLEEPVITK DIYEVAVSLI 360
QMFDDLDMKE SGNKAWSGAQ FVLERSSVLV FLPGLGEINY MHELLTSLVH KRLQVYPLHS 420
SVALEEQNNV FLSPVPGYRK IILSTNIAES SVTVPDVKYV IDFCLTRTLV CDEDTNYQSL 480
RLSWASKTSC NQRKGRAGRV SRGYCYRLVH KDFWDNSIPD HVVPEMLRCP LGSTILKVKL 540
LDMGEPRALL ATALSPPGLS DIERTILLLK EVGALAVSGQ REDENPHDGE LTFLGRVLAQ 600
LPVNQQLGKL IVLGHVFGCL DECLIIAAAL SLKNFFAMPF RQHLDGYRNK VNFSGSSKSD 660
CIALVEAFKT WKACRQTGEL RYPKDELNWG RLNYIQIKRI REVAELYEEL KTRISQFNMH 720
VDSRRPVMDQ EYIYKQRFIL QVVLAGAFYP NYFTFGQPDE EMAVRELAGK DPKTTVVLKH 780
IPPYGFLYYK QLQSLFRQCG QVKSIVFDGA KAFVEFSRNP TERFKTLPAV YMAIKMSQLK 840
VSLELSVHSA EEIEGKVQGM NVSKLRNTRV NVDFQKQTVD PMQVSFNTSD RSQTVTDLLL 900
TIDVTEVVEV GHFWGYRIDE NNSEILKKLT AEINQLTLVP LPTHPHPDLV CLAPFADFDK 960
QRYFRAQVLY VSGNSAEVFF VDYGNKSHVD LHLLMEIPCQ FLELPFQALE FKICKMRPSA 1020
KSLVCGKHWS DGASQWFASL VSGCTLLVKV FSVVHSVLHV DVYQYSGVQD AINIRDVLIQ 1080
QGYAELTEES YESKQSHEVL KGLFSKSVEN MTDGSVPFPM KDDEKYLIRI LLESFSTNKL 1140
GTPNCKAELH GPFNPYELKC HSLTRISKFR CVWIEKESIN SVIISDAPED LHQRMLVAAS 1200
LSINATGSTM LLRETSLMPH IPGLPALLSM LFAPVIELRI DQNGKYYTGV LCGLGWNPAT 1260
GASILPEHDM ELAFDVQFSV EDVVEVNILR AAINKLVCDG PNGCKCLGPE RVAQLQDIAR 1320
QKLLGLFCQS KPREKIVPKW HEKPYEWNQV DPKLVMEQAD RESSRGKNTF LYQLHKLVVL 1380
GT 1382 
Gene Ontology
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0071547; C:piP-body; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0043046; P:DNA methylation involved in gamete generation; ISS:UniProtKB.
 GO:0009566; P:fertilization; ISS:UniProtKB.
 GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
 GO:0007140; P:male meiosis; ISS:UniProtKB.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0034587; P:piRNA metabolic process; ISS:UniProtKB.
 GO:0007283; P:spermatogenesis; ISS:UniProtKB. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR002999; Tudor. 
Pfam
 PF00270; DEAD
 PF04408; HA2
 PF00271; Helicase_C
 PF00567; TUDOR 
SMART
 SM00487; DEXDc
 SM00847; HA2
 SM00490; HELICc
 SM00333; TUDOR 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS50304; TUDOR 
PRINTS