UniProt Accession

 URIC_MOUSE; P25688 

Genbank Protein ID

 M27695; BC019771 

Genbank Nucleotide ID

 AAA40538.1; AAH19771.1 

Protein Name

 Uricase 

Protein Synonyms/Alias

 Urate oxidase 

Gene Name

 Uox 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
10	HYHDNYGKNDEVEFV	acetylation	[1, 2, 3, 4, 5]
10	HYHDNYGKNDEVEFV	succinylation	[4]
10	HYHDNYGKNDEVEFV	ubiquitination	[6]
23	FVRTGYGKDMVKVLH	acetylation	[2, 3, 4, 5]
23	FVRTGYGKDMVKVLH	succinylation	[4]
23	FVRTGYGKDMVKVLH	ubiquitination	[6]
27	GYGKDMVKVLHIQRD	acetylation	[2, 3]
27	GYGKDMVKVLHIQRD	ubiquitination	[6]
36	LHIQRDGKYHSIKEV	acetylation	[1, 2, 3, 5, 7, 8]
54	VQLTLRSKKDYLHGD	acetylation	[3]
55	QLTLRSKKDYLHGDN	acetylation	[7]
55	QLTLRSKKDYLHGDN	ubiquitination	[6]
72	IIPTDTIKNTVHVLA	acetylation	[2, 5]
72	IIPTDTIKNTVHVLA	ubiquitination	[6]
80	NTVHVLAKLRGIRNI	acetylation	[2, 4, 5]
80	NTVHVLAKLRGIRNI	succinylation	[4]
80	NTVHVLAKLRGIRNI	ubiquitination	[6]
118	YVEEVPWKRFEKNGI	acetylation	[2, 7]
118	YVEEVPWKRFEKNGI	ubiquitination	[6]
122	VPWKRFEKNGIKHVH	acetylation	[2, 7]
126	RFEKNGIKHVHAFIH	acetylation	[2]
158	PVIHSGIKDLKVLKT	acetylation	[2]
158	PVIHSGIKDLKVLKT	ubiquitination	[6]
161	HSGIKDLKVLKTTQS	acetylation	[2]
164	IKDLKVLKTTQSGFE	acetylation	[7]
164	IKDLKVLKTTQSGFE	ubiquitination	[6]
175	SGFEGFLKDQFTTLP	acetylation	[2]
175	SGFEGFLKDQFTTLP	ubiquitination	[6]
185	FTTLPEVKDRCFATQ	acetylation	[2, 7]
185	FTTLPEVKDRCFATQ	ubiquitination	[6]
196	FATQVYCKWRYQRRD	acetylation	[2]
196	FATQVYCKWRYQRRD	ubiquitination	[6]
220	VRDIVLQKFAGPYDK	acetylation	[2, 7]
220	VRDIVLQKFAGPYDK	ubiquitination	[6]
227	KFAGPYDKGEYSPSV	acetylation	[2, 7]
227	KFAGPYDKGEYSPSV	ubiquitination	[6]
277	SKMGLINKEEVLLPL	acetylation	[1, 2, 3, 4, 5, 7, 8]
277	SKMGLINKEEVLLPL	succinylation	[4]
290	PLDNPYGKITGTVKR	acetylation	[1, 4, 5, 7]
290	PLDNPYGKITGTVKR	succinylation	[4]
296	GKITGTVKRKLPSRL	acetylation	[3]

Reference

 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [7] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [8] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199] 

Functional Description

 Catalyzes the oxidation of uric acid to 5- hydroxyisourate, which is further processed to form (S)-allantoin. 

Sequence Annotation

 REGION 68 69 Substrate binding (By similarity).
 REGION 234 235 Substrate binding (By similarity).
 MOTIF 301 303 Microbody targeting signal (Potential).
 ACT_SITE 187 187 Charge relay system (By similarity).
 ACT_SITE 235 235 Charge relay system (By similarity).
 BINDING 187 187 Substrate (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 36 36 N6-acetyllysine.
 MOD_RES 39 39 Phosphoserine.
 MOD_RES 55 55 N6-acetyllysine.
 MOD_RES 118 118 N6-acetyllysine.
 MOD_RES 122 122 N6-acetyllysine.
 MOD_RES 164 164 N6-acetyllysine.
 MOD_RES 185 185 N6-acetyllysine.
 MOD_RES 220 220 N6-acetyllysine.
 MOD_RES 227 227 N6-acetyllysine.
 MOD_RES 231 231 Phosphoserine.
 MOD_RES 277 277 N6-acetyllysine.
 MOD_RES 290 290 N6-acetyllysine.
 MOD_RES 301 301 Phosphoserine.  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Oxidoreductase; Peroxisome; Phosphoprotein; Purine metabolism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 303 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005777; C:peroxisome; TAS:MGI.
 GO:0004846; F:urate oxidase activity; TAS:MGI.
 GO:0006144; P:purine nucleobase metabolic process; TAS:MGI.
 GO:0019628; P:urate catabolic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR002042; Uricase.
 IPR019842; Uricase_CS. 

Pfam

 PF01014; Uricase 

SMART

  

PROSITE

 PS00366; URICASE 

PRINTS

 PR00093; URICASE.