CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000385
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Actin-related protein 2/3 complex subunit 1B 
Protein Synonyms/Alias
 Arp2/3 complex 41 kDa subunit; p41-ARC 
Gene Name
 ARPC1B 
Gene Synonyms/Alias
 ARC41 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18ISCHAWNKDRTQIAIubiquitination[1]
37HEVHIYEKSGAKWTKubiquitination[1]
44KSGAKWTKVHELKEHacetylation[2]
49WTKVHELKEHNGQVTubiquitination[1]
82NAYVWTLKGRTWKPTacetylation[2]
82NAYVWTLKGRTWKPTubiquitination[1, 3]
87TLKGRTWKPTLVILRubiquitination[1]
110RWAPNENKFAVGSGSubiquitination[1, 3, 4, 5]
174RIFSAYIKEVEERPAacetylation[6, 7]
174RIFSAYIKEVEERPAubiquitination[1]
289GGRLDVPKQSSQRGLubiquitination[1]
308RFQNLDKKASSEGGTubiquitination[1, 7]
326AGLDSLHKNSVSQISubiquitination[1, 5]
339ISVLSGGKAKCSQFCubiquitination[5]
341VLSGGKAKCSQFCTTubiquitination[1]
360GMSIWDVKSLESALKubiquitination[5]
367KSLESALKDLKIK**ubiquitination[1, 5, 7]
370ESALKDLKIK*****ubiquitination[5]
372ALKDLKIK*******ubiquitination[5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. 
Sequence Annotation
 REPEAT 6 45 WD 1.
 REPEAT 50 89 WD 2.
 REPEAT 94 135 WD 3.
 REPEAT 140 179 WD 4.
 REPEAT 242 280 WD 5.
 REPEAT 324 367 WD 6.
 MOD_RES 82 82 N6-acetyllysine.
 MOD_RES 310 310 Phosphoserine (By similarity).  
Keyword
 Acetylation; Actin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 372 AA 
Protein Sequence
MAYHSFLVEP ISCHAWNKDR TQIAICPNNH EVHIYEKSGA KWTKVHELKE HNGQVTGIDW 60
APESNRIVTC GTDRNAYVWT LKGRTWKPTL VILRINRAAR CVRWAPNENK FAVGSGSRVI 120
SICYFEQEND WWVCKHIKKP IRSTVLSLDW HPNNVLLAAG SCDFKCRIFS AYIKEVEERP 180
APTPWGSKMP FGELMFESSS SCGWVHGVCF SASGSRVAWV SHDSTVCLAD ADKKMAVATL 240
ASETLPLLAL TFITDNSLVA AGHDCFPVLF TYDAAAGMLS FGGRLDVPKQ SSQRGLTARE 300
RFQNLDKKAS SEGGTAAGAG LDSLHKNSVS QISVLSGGKA KCSQFCTTGM DGGMSIWDVK 360
SLESALKDLK IK 372 
Gene Ontology
 GO:0005885; C:Arp2/3 protein complex; TAS:ProtInc.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005200; F:structural constituent of cytoskeleton; TAS:ProtInc.
 GO:0006928; P:cellular component movement; TAS:ProtInc.
 GO:0030833; P:regulation of actin filament polymerization; IEA:InterPro. 
Interpro
 IPR017383; ARPC2/3_su1.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS