UniProt Accession

 IF5_YEAST; P38431; D6W450 

Genbank Protein ID

 L10840; Z71255; Z68111; Z73616; BK006949 

Genbank Nucleotide ID

 CAA94989.1; CAA92145.1; CAA97991.1; DAA11466.1 

Protein Name

 Eukaryotic translation initiation factor 5 

Protein Synonyms/Alias

 eIF-5 

Gene Name

 TIF5 

Gene Synonyms/Alias

 YPR041W; YP3085.05 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
113	ETEIIITKDNDLVRD	acetylation	[1]
142	KLSSFILKNPPDSVS	acetylation	[1]
178	ISDIAQGKSQNAPSD	ubiquitination	[2]
274	PSDVELYKKAAELDV	acetylation	[1]
275	SDVELYKKAAELDVL	ubiquitination	[2]
322	LVTPEYEKNFMGGIE	acetylation	[1]
322	LVTPEYEKNFMGGIE	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). eIF-5 is essential for cell viability. 

Sequence Annotation

 DOMAIN 241 402 W2.
 NP_BIND 27 34 GTP (Potential).
 MOD_RES 170 170 Phosphoserine.
 MOD_RES 172 172 Phosphoserine.
 MOD_RES 191 191 Phosphothreonine.
 MOD_RES 228 228 Phosphoserine.
 MOD_RES 317 317 Phosphothreonine.
 MOD_RES 397 397 Phosphoserine.  

Keyword

 3D-structure; Alternative initiation; Complete proteome; Direct protein sequencing; GTP-binding; Initiation factor; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 405 AA 

Protein Sequence

Gene Ontology

 GO:0022627; C:cytosolic small ribosomal subunit; IPI:SGD.
 GO:0043614; C:multi-eIF complex; IDA:SGD.
 GO:0005092; F:GDP-dissociation inhibitor activity; IDA:SGD.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0005096; F:GTPase activator activity; IPI:SGD.
 GO:0003743; F:translation initiation factor activity; ISS:SGD.
 GO:0042256; P:mature ribosome assembly; IMP:SGD.
 GO:0045947; P:negative regulation of translational initiation; IMP:SGD.
 GO:0016070; P:RNA metabolic process; IEA:InterPro. 

Interpro

 IPR016024; ARM-type_fold.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR002735; Transl_init_fac_IF2/IF5.
 IPR016189; Transl_init_fac_IF2/IF5_N.
 IPR016190; Transl_init_fac_IF2/IF5_Zn-bd.
 IPR003307; W2_domain. 

Pfam

 PF01873; eIF-5_eIF-2B
 PF02020; W2 

SMART

 SM00653; eIF2B_5
 SM00515; eIF5C 

PROSITE

 PS51363; W2 

PRINTS