CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-034207
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Long-chain-fatty-acid--CoA ligase 1 
Protein Synonyms/Alias
  
Gene Name
 Acsl1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
52ATRPKALKPPCDLSMubiquitination[1]
81SAVLEDDKLLVYYYDubiquitination[1]
114GPCLGSRKPNQPYEWubiquitination[1]
203LSVIFADKPEKAKLLubiquitination[1]
208ADKPEKAKLLLEGVEacetylation[2, 3]
208ADKPEKAKLLLEGVEubiquitination[1]
217LLEGVENKLTPCLKIacetylation[2, 4]
217LLEGVENKLTPCLKIubiquitination[1]
241DLVERGKKCGVEIISubiquitination[1]
303NDCSGFIKATESALTacetylation[3]
357RLLMDDLKVLQPTIFacetylation[2]
357RLLMDDLKVLQPTIFubiquitination[1]
387GQANTSLKRWLLDFAacetylation[2, 3]
387GQANTSLKRWLLDFAubiquitination[1]
396WLLDFASKRKEAELRubiquitination[1]
415RNNSLWDKLIFHKIQubiquitination[1]
420WDKLIFHKIQSSLGGubiquitination[1]
428IQSSLGGKVRLMITGubiquitination[1]
512GEGEVCVKGANVFKGubiquitination[1]
518VKGANVFKGYLKDPAacetylation[3]
518VKGANVFKGYLKDPAubiquitination[1]
522NVFKGYLKDPARTAEubiquitination[1]
533RTAEALDKDGWLHTGacetylation[3]
533RTAEALDKDGWLHTGubiquitination[1]
544LHTGDIGKWLPNGTLacetylation[3]
544LHTGDIGKWLPNGTLphosphoglycerylation[5]
552WLPNGTLKIIDRKKHacetylation[2, 3]
552WLPNGTLKIIDRKKHphosphoglycerylation[5]
552WLPNGTLKIIDRKKHubiquitination[1]
562DRKKHIFKLAQGEYIubiquitination[1]
629FEELCRNKDINKAILacetylation[3]
633CRNKDINKAILDDLLacetylation[3]
633CRNKDINKAILDDLLubiquitination[1]
641AILDDLLKLGKEAGLubiquitination[1]
644DDLLKLGKEAGLKPFacetylation[3]
644DDLLKLGKEAGLKPFubiquitination[1]
649LGKEAGLKPFEQVKGacetylation[3, 6]
649LGKEAGLKPFEQVKGsuccinylation[6]
655LKPFEQVKGIAVHPEubiquitination[1]
676GLLTPTLKAKRPELRubiquitination[1]
698DELYATIKI******ubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [5] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]
 [6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 699 AA 
Protein Sequence
MEVHELFRYF RMPELIDIRQ YVRTLPTNTL MGFGAFAALT TFWYATRPKA LKPPCDLSMQ 60
SVEIAGTTDG IRRSAVLEDD KLLVYYYDDV RTMYDGFQRG IQVSNNGPCL GSRKPNQPYE 120
WISYKEVAEL AECIGSGLIQ KGFKPCSEQF IGLFSQNRPE WVIVEQGCFS YSMVVVPLYD 180
TLGADAITYI VNKAELSVIF ADKPEKAKLL LEGVENKLTP CLKIIVIMDS YGSDLVERGK 240
KCGVEIISLK ALEDLGRVNR VKPKPPEPED LAIICFTSGT TGNPKGAMIT HQNIINDCSG 300
FIKATESALT LNASDTQISY LPLAHMYEQQ LQCVMLCHGA KIGFFQGDIR LLMDDLKVLQ 360
PTIFPVVPRL LNRMFDRIFG QANTSLKRWL LDFASKRKEA ELRSGIVRNN SLWDKLIFHK 420
IQSSLGGKVR LMITGAAPVS ATVLTFLRTA LGCQFYEGYG QTECTAGCCL SLPGDWTAGH 480
VGAPMPCNYV KLVDVEEMNY LASKGEGEVC VKGANVFKGY LKDPARTAEA LDKDGWLHTG 540
DIGKWLPNGT LKIIDRKKHI FKLAQGEYIA PEKIENIYLR SEAVAQVFVH GESLQAFLIA 600
VVVPDVESLP SWAQKRGLQG SFEELCRNKD INKAILDDLL KLGKEAGLKP FEQVKGIAVH 660
PELFSIDNGL LTPTLKAKRP ELRNYFRSQI DELYATIKI 699 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0033211; P:adiponectin-mediated signaling pathway; IMP:MGI.
 GO:0071902; P:positive regulation of protein serine/threonine kinase activity; IMP:MGI. 
Interpro
 IPR020845; AMP-binding_CS.
 IPR000873; AMP-dep_Synth/Lig. 
Pfam
 PF00501; AMP-binding 
SMART
  
PROSITE
 PS00455; AMP_BINDING 
PRINTS