CPLM-021234

Genbank Nucleotide ID

Cleavage stimulation factor subunit 2 tau variant

Protein Synonyms/Alias

CF-1 64 kDa subunit tau variant; Cleavage stimulation factor 64 kDa subunit tau variant; CSTF 64 kDa subunit tau variant; TauCstF-64

Homo sapiens (Human)

Position	Peptide	Type	References
55	VYDRETGKPKGYGFC	ubiquitination	[1]
145	EQMFELMKQMKLCVQ	ubiquitination	[1, 2]
148	FELMKQMKLCVQNSH	ubiquitination	[1, 2, 3, 4, 5, 6]
189	MDPEIALKILHRKIH	ubiquitination	[1, 2, 3, 5, 6, 7]
194	ALKILHRKIHVTPLI	ubiquitination	[1, 3, 5, 6, 8]
611	ILKEQIQKSTGAS**	ubiquitination	[1]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

May play a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. Directly involved in the binding to pre-mRNAs (By similarity).

DOMAIN 16 94 RRM.
REPEAT 418 422 1-1.
REPEAT 423 427 1-2; approximate.
REPEAT 428 432 1-3; approximate.
REPEAT 433 437 1-4; approximate.
REPEAT 438 442 1-5; approximate.
REPEAT 443 447 1-6.
REPEAT 448 452 1-7; approximate.
REPEAT 453 457 1-8; approximate.
REPEAT 458 462 1-9; approximate.
REPEAT 505 509 2-1.
REPEAT 510 514 2-2.
REPEAT 515 519 2-3.
REPEAT 520 524 2-4.
REPEAT 525 529 2-5; approximate.
REPEAT 530 534 2-6.
REPEAT 535 539 2-7; approximate.
REPEAT 540 544 2-8; approximate.
REPEAT 545 549 2-9; approximate.
REGION 418 462 9 X 5 AA tandem repeats of M-E-T-R-[AG].
REGION 505 549 9 X 5 AA tandem repeats of G-[AT]-G-[MI]-

Complete proteome; mRNA processing; Nucleus; Reference proteome; Repeat; RNA-binding.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005622; C:intracellular; IDA:LIFEdb.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.

IPR025742; CSTF2_hinge.
IPR026896; CSTF_C.
IPR012677; Nucleotide-bd_a/b_plait.
IPR000504; RRM_dom.

PF14327; CSTF2_hinge
PF14304; CSTF_C
PF00076; RRM_1