CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012232
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytoskeleton-associated protein 5 
Protein Synonyms/Alias
 Colonic and hepatic tumor overexpressed gene protein; Ch-TOG 
Gene Name
 CKAP5 
Gene Synonyms/Alias
 KIAA0097 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
150ACIETLRKALSEFGSubiquitination[1]
438TLPKSLLKPFCAALLubiquitination[1]
689VLDGLVDKIGDVKCGubiquitination[1]
906GELPTALKGRLNDSNubiquitination[1]
1523PVLIPEPKIRAVSPHubiquitination[1]
1835DEKSSKAKVNDFLAEubiquitination[1]
1988STDMLHSKLSQLRESubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. 
Sequence Annotation
 REPEAT 159 197 HEAT 1.
 REPEAT 356 394 HEAT 2.
 REPEAT 434 472 HEAT 3.
 REPEAT 750 788 HEAT 4.
 REPEAT 855 893 HEAT 5.
 REPEAT 936 974 HEAT 6.
 REPEAT 1013 1051 HEAT 7.
 REPEAT 1284 1322 HEAT 8.
 REPEAT 1324 1357 HEAT 9.
 REPEAT 1361 1399 HEAT 10.
 MOD_RES 48 48 N6-acetyllysine.
 MOD_RES 816 816 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Mitosis; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2032 AA 
Protein Sequence
MGDDSEWLKL PVDQKCEHKL WKARLSGYEE ALKIFQKIKD EKSPEWSKFL GLIKKFVTDS 60
NAVVQLKGLE AALVYVENAH VAGKTTGEVV SGVVSKVFNQ PKAKAKELGI EICLMYIEIE 120
KGEAVQEELL KGLDNKNPKI IVACIETLRK ALSEFGSKII LLKPIIKVLP KLFESREKAV 180
RDEAKLIAVE IYRWIRDALR PPLQNINSVQ LKELEEEWVK LPTSAPRPTR FLRSQQELEA 240
KLEQQQSAGG DAEGGGDDGD EVPQIDAYEL LEAVEILSKL PKDFYDKIEA KKWQERKEAL 300
ESVEVLIKNP KLEAGDYADL VKALKKVVGK DTNVMLVALA AKCLTGLAVG LRKKFGQYAG 360
HVVPTILEKF KEKKPQVVQA LQEAIDAIFL TTTLQNISED VLAVMDNKNP TIKQQTSLFI 420
ARSFRHCTAS TLPKSLLKPF CAALLKHIND SAPEVRDAAF EALGTALKVV GEKAVNPFLA 480
DVDKLKLDKI KECSEKVELI HGKKAGLAAD KKEFKPLPGR TAASGAAGDK DTKDISAPKP 540
GPLKKAPAAK AGGPPKKGKP AAPGGAGNTG TKNKKGLETK EIVEPELSIE VCEEKASAVL 600
PPTCIQLLDS SNWKERLACM EEFQKAVELM DRTEMPCQAL VRMLAKKPGW KETNFQVMQM 660
KLHIVALIAQ KGNFSKTSAQ VVLDGLVDKI GDVKCGNNAK EAMTAIAEAC MLPWTAEQVV 720
SMAFSQKNPK NQSETLNWLS NAIKEFGFSG LNVKAFISNV KTALAATNPA VRTAAITLLG 780
VMYLYVGPSL RMFFEDEKPA LLSQIDAEFE KMQGQSPPAP TRGISKHSTS GTDEGEDGDE 840
PDDGSNDVVD LLPRTEISDK ITSELVSKIG DKNWKIRKEG LDEVAGIIND AKFIQPNIGE 900
LPTALKGRLN DSNKILVQQT LNILQQLAVA MGPNIKQHVK NLGIPIITVL GDSKNNVRAA 960
ALATVNAWAE QTGMKEWLEG EDLSEELKKE NPFLRQELLG WLAEKLPTLR STPTDLILCV 1020
PHLYSCLEDR NGDVRKKAQD ALPFFMMHLG YEKMAKATGK LKPTSKDQVL AMLEKAKVNM 1080
PAKPAPPTKA TSKPMGGSAP AKFQPASAPA EDCISSSTEP KPDPKKAKAP GLSSKAKSAQ 1140
GKKMPSKTSL KEDEDKSGPI FIVVPNGKEQ RMKDEKGLKV LKWNFTTPRD EYIEQLKTQM 1200
SSCVAKWLQD EMFHSDFQHH NKALAVMVDH LESEKEGVIG CLDLILKWLT LRFFDTNTSV 1260
LMKALEYLKL LFTLLSEEEY HLTENEASSF IPYLVVKVGE PKDVIRKDVR AILNRMCLVY 1320
PASKMFPFIM EGTKSKNSKQ RAECLEELGC LVESYGMNVC QPTPGKALKE IAVHIGDRDN 1380
AVRNAALNTI VTVYNVHGDQ VFKLIGNLSE KDMSMLEERI KRSAKRPSAA PIKQVEEKPQ 1440
RAQNISSNAN MLRKGPAEDM SSKLNQARSM SGHPEAAQMV RREFQLDLDE IENDNGTVRC 1500
EMPELVQHKL DDIFEPVLIP EPKIRAVSPH FDDMHSNTAS TINFIISQVA SGDINTSIQA 1560
LTQIDEVLRQ EDKAEAMSGH IDQFLIATFM QLRLIYNTHM ADEKLEKDEI IKLYSCIIGN 1620
MISLFQIESL AREASTGVLK DLMHGLITLM LDSRIEDLEE GQQVIRSVNL LVVKVLEKSD 1680
QTNILSALLV LLQDSLLATA SSPKFSELVM KCLWRMVRLL PDTINSINLD RILLDIHIFM 1740
KVFPKEKLKQ CKSEFPIRTL KTLLHTLCKL KGPKSCMCLP QILDHLTMID NKNESELEAH 1800
LCRMMKHSMD QTGSKSDKET EKGASRIDEK SSKAKVNDFL AEIFKKIGSK ENTKEGLAEL 1860
YEYKKKYSDA DIEPFLKNSS QFFQSYVERG LRVIEMEREG KGRISTSTGI SPQMEVTCVP 1920
TPTSTVSSIG NTNGEEVGPS VYLERLKILR QRCGLDNTKQ DDRPPLTSLL SKPAVPTVAS 1980
STDMLHSKLS QLRESREQHQ HSDLDSNQTH SSGTVTSSSS TANIDDLKKR LERIKSSRK 2039 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000922; C:spindle pole; IDA:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051297; P:centrosome organization; IMP:HGNC.
 GO:0030951; P:establishment or maintenance of microtubule cytoskeleton polarity; IMP:HGNC.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0050658; P:RNA transport; ISS:HGNC.
 GO:0007051; P:spindle organization; IMP:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR024395; CLASP_N_dom.
 IPR000357; HEAT.
 IPR021133; HEAT_type_2. 
Pfam
 PF12348; CLASP_N
 PF02985; HEAT 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS