CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000497
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phospholipase A1-Ibeta2, chloroplastic 
Protein Synonyms/Alias
  
Gene Name
 At4g16820; dl4435w, FCAALL.42 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
43SPARKTNKEHLRNLEubiquitination[1]
Reference
 [1] Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants.
 Maor R, Jones A, Nühse TS, Studholme DJ, Peck SC, Shirasu K.
 Mol Cell Proteomics. 2007 Apr;6(4):601-10. [PMID: 17272265
Functional Description
 Acylhydrolase that catalyzes the hydrolysis of phosphatidylcholine at the sn-1 position. Has a strong galactolipase activity toward digalactosyldiacylglycerol (DGDG). Hydrolyzes triacylglycerol (TAG), but has a low activity toward phosphatidylcholine (PC) and monogalactosyldiacylglycerol (MGDG). 
Sequence Annotation
 ACT_SITE 327 327 Acyl-ester intermediate (Potential).
 ACT_SITE 384 384 Charge relay system (By similarity).
 ACT_SITE 451 451 Charge relay system (By similarity).
 CROSSLNK 43 43 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 490 490 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Chloroplast; Complete proteome; Hydrolase; Isopeptide bond; Lipid degradation; Lipid metabolism; Plastid; Reference proteome; Transit peptide; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 517 AA 
Protein Sequence
MQTLTPNADI FHAKRRRFTC NTHTSTLIPT KPLSVSPARK TNKEHLRNLE NVLRTSSNSI 60
DHIENVTSRQ EKTTKNTSTS SLLGGLNLAR IWPQMKAAVD EMSPKNLKRL QRLLSKSSEE 120
RSPKSKLGSK WRELHGLNNW AGLLDPLDEN LRRELVRYGE FVQAAYHAFH SDPEGSPRHV 180
ALPDGSFKVT KSLYATSSVR LPKWIDDVAP DLRWMTKQTS WVGYVAVCDD PREIRRMGRR 240
EIVIALRGTA TLLEWSENFR PNLVSMPEPK PDQSDPTRPK VECGFNSLYT TGDQHAPSLA 300
ESLVGEISRL VELYAGEELS ISVTGHSLGA AIALLAADDI AERVPHAPPV AVFSFGGPRV 360
GNREFADRLD SKGVKVLRVV NSQDVVTKVP GIFADNDKQG QSRNNGRSPG GIMEMVERNN 420
PWAYSHVGAE LRVDMKMSPY LKPNADVACC HDLEAYLHLV DGFLASNCPF RANAKRSLRK 480
LLDEQRSNVK VLYTGKSLRL NRTFHDNGDV LPSPSSS 517 
Gene Ontology
 GO:0009507; C:chloroplast; IDA:TAIR.
 GO:0047714; F:galactolipase activity; IDA:TAIR.
 GO:0008970; F:phosphatidylcholine 1-acylhydrolase activity; IDA:TAIR.
 GO:0004806; F:triglyceride lipase activity; IDA:TAIR.
 GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW. 
Interpro
 IPR002921; Lipase_3. 
Pfam
 PF01764; Lipase_3 
SMART
  
PROSITE
 PS00120; LIPASE_SER 
PRINTS