UniProt Accession

 ZWINT_HUMAN; O95229; A6NNV6; Q0D2I3; Q9BWD0 

Genbank Protein ID

 AF067656; AC010996; BC000411; BC020979; BC110399 

Genbank Nucleotide ID

 AAC78629.1; AAH00411.1; AAH20979.1; AAI10400.1 

Protein Name

 ZW10 interactor 

Protein Synonyms/Alias

 ZW10-interacting protein 1; Zwint-1 

Gene Name

 ZWINT 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
48	EFVVDSQKKDKLLCS	ubiquitination	[1, 2, 3]
49	FVVDSQKKDKLLCSQ	ubiquitination	[3]
51	VDSQKKDKLLCSQLQ	ubiquitination	[1, 3]
93	RQKAIAAKEQWKELK	ubiquitination	[3, 4, 5, 6]
97	IAAKEQWKELKATYR	ubiquitination	[3, 6]
100	KEQWKELKATYREHV	ubiquitination	[1, 3, 6, 7]
111	REHVEAIKIGLTKAL	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9, 10]
116	AIKIGLTKALTQMEE	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12]
141	AFEQLQAKKQMAMEK	ubiquitination	[1, 3, 7, 9]
162	QWQLQQEKHLQHLAE	ubiquitination	[3]
191	ELDRVFQKLGNLKQQ	ubiquitination	[3]
196	FQKLGNLKQQAEQER	ubiquitination	[1, 3, 7, 9]
205	QAEQERDKLQRYQTF	ubiquitination	[3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [11] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [12] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase. 

Sequence Annotation

 REGION 80 155 Interaction with NDC80 and ZW10.  

Keyword

 Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Kinetochore; Mitosis; Nucleus; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 277 AA 

Protein Sequence

Gene Ontology

 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0000776; C:kinetochore; IDA:BHF-UCL.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051649; P:establishment of localization in cell; IDA:HGNC.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0007093; P:mitotic cell cycle checkpoint; IDA:HGNC.
 GO:0000070; P:mitotic sister chromatid segregation; IDA:HGNC.
 GO:0048015; P:phosphatidylinositol-mediated signaling; NAS:UniProtKB.
 GO:0007051; P:spindle organization; NAS:UniProtKB. 

Interpro

  

Pfam

  

SMART

  

PROSITE

  

PRINTS