CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005389
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Intracellular protein transport protein USO1 
Protein Synonyms/Alias
 Int-1 
Gene Name
 USO1 
Gene Synonyms/Alias
 INT1; YDL058W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
119YPSPLVMKQEKEQVDmethylation[1]
972KSLANNYKDMQAENEubiquitination[2]
1062QISLLKEKLETATTAubiquitination[2]
1367IKNQAFEKERKLLNEubiquitination[2]
1656STQQKAQKSEEERRAacetylation[3]
Reference
 [1] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Required for protein transport from the ER to the Golgi complex. 
Sequence Annotation
 REPEAT 45 89 ARM 1.
 REPEAT 127 170 ARM 2.
 REPEAT 173 213 ARM 3.
 REPEAT 215 260 ARM 4.
 REPEAT 261 312 ARM 5.
 REPEAT 314 362 ARM 6.
 REPEAT 363 429 ARM 7.
 REPEAT 431 512 ARM 8.
 REPEAT 543 584 ARM 9.
 REPEAT 586 630 ARM 10.
 REGION 1 724 Globular head.
 REGION 465 487 Charged (hyper-hydrophilic).
 REGION 991 1790 Dispensable for the protein function.
 MOD_RES 1770 1770 Phosphoserine.  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Direct protein sequencing; Endoplasmic reticulum; Golgi apparatus; Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1790 AA 
Protein Sequence
MDIIQGLIQQ PKIQSVDETI PTLCDRVENS TLISDRRSAV LGLKAFSRQY RESVIASGLK 60
PLLNTLKRDY MDEDSVKAIL ETILILFIRG DGHDDLTRGW ISQQSRLQNG KYPSPLVMKQ 120
EKEQVDQFSL WIADALTQSE DLIHLLVEFW EIDNFHIRLY TIQLLEAVMA TRPLKARSAL 180
ISLPTSISTM VSLLDDMHEP IRDEAILLLM AVVNDSPHVQ KLVAFENIFE RLFSIIEEEG 240
GLRGSLVVND CLSLINNILK YNTSNQTLFL ETGNLPKLAH LLSEPISQDE VFFWNDQRIV 300
NINTALDIVS LTVEPGNTVT TKHQNALLDS SVLMVVLRLA FFHNIPKKVR PVALLTAANM 360
VRSNEHAQLE FSKIDVPYFD PSLPVNSTAN GGPIKLIPVV SILINWMLYA NSVHTFDTRV 420
ACSRLLKAYF MDNFDLQRDF LLKQVQLCNN STNNVGDNAK ENGGSNKSDK ESDSDKDTDG 480
KDGTEYEGSF KANLFEVLLN YDAELNLNPF KLFFTTDIFM FFFQQDHKYS EELREITRNV 540
TTGNDLEDEE PLKAIQTISE LLTTSLTAAD IRIPISYLTF LIYWLFGDFK ATNDFLSDKS 600
VIKSLLSFSY QIQDEDVTIK CLVTMLLGVA YEFSSKESPF PRKEYFEFIT KTLGKDNYAS 660
RIKQFKKDSY FSKVDMNEDS ILTPELDETG LPKVYFSTYF IQLFNENIYR IRTALSHDPD 720
EEPINKISFE EVEKLQRQCT KLKGEITSLQ TETESTHENL TEKLIALTNE HKELDEKYQI 780
LNSSHSSLKE NFSILETELK NVRDSLDEMT QLRDVLETKD KENQTALLEY KSTIHKQEDS 840
IKTLEKGLET ILSQKKKAED GINKMGKDLF ALSREMQAVE ENCKNLQKEK DKSNVNHQKE 900
TKSLKEDIAA KITEIKAINE NLEEMKIQCN NLSKEKEHIS KELVEYKSRF QSHDNLVAKL 960
TEKLKSLANN YKDMQAENES LIKAVEESKN ESSIQLSNLQ NKIDSMSQEK ENFQIERGSI 1020
EKNIEQLKKT ISDLEQTKEE IISKSDSSKD EYESQISLLK EKLETATTAN DENVNKISEL 1080
TKTREELEAE LAAYKNLKNE LETKLETSEK ALKEVKENEE HLKEEKIQLE KEATETKQQL 1140
NSLRANLESL EKEHEDLAAQ LKKYEEQIAN KERQYNEEIS QLNDEITSTQ QENESIKKKN 1200
DELEGEVKAM KSTSEEQSNL KKSEIDALNL QIKELKKKNE TNEASLLESI KSVESETVKI 1260
KELQDECNFK EKEVSELEDK LKASEDKNSK YLELQKESEK IKEELDAKTT ELKIQLEKIT 1320
NLSKAKEKSE SELSRLKKTS SEERKNAEEQ LEKLKNEIQI KNQAFEKERK LLNEGSSTIT 1380
QEYSEKINTL EDELIRLQNE NELKAKEIDN TRSELEKVSL SNDELLEEKQ NTIKSLQDEI 1440
LSYKDKITRN DEKLLSIERD NKRDLESLKE QLRAAQESKA KVEEGLKKLE EESSKEKAEL 1500
EKSKEMMKKL ESTIESNETE LKSSMETIRK SDEKLEQSKK SAEEDIKNLQ HEKSDLISRI 1560
NESEKDIEEL KSKLRIEAKS GSELETVKQE LNNAQEKIRI NAEENTVLKS KLEDIERELK 1620
DKQAEIKSNQ EEKELLTSRL KELEQELDST QQKAQKSEEE RRAEVRKFQV EKSQLDEKAM 1680
LLETKYNDLV NKEQAWKRDE DTVKKTTDSQ RQEIEKLAKE LDNLKAENSK LKEANEDRSE 1740
IDDLMLLVTD LDEKNAKYRS KLKDLGVEIS SDEEDDEEDD EEDEEEGQVA 1790 
Gene Ontology
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0012507; C:ER to Golgi transport vesicle membrane; IDA:SGD.
 GO:0000139; C:Golgi membrane; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:SGD.
 GO:0048211; P:Golgi vesicle docking; IDA:SGD.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0035493; P:SNARE complex assembly; IMP:SGD.
 GO:0048280; P:vesicle fusion with Golgi apparatus; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR006955; Uso1_p115_C.
 IPR024095; Vesicle_P115-like.
 IPR006953; Vesicle_Uso1_P115_head. 
Pfam
 PF04871; Uso1_p115_C
 PF04869; Uso1_p115_head 
SMART
  
PROSITE
 PS50176; ARM_REPEAT 
PRINTS