UniProt Accession

 PNC1_YEAST; P53184; D6VUA2 

Genbank Protein ID

 Z72559; AY558481; BK006941 

Genbank Nucleotide ID

 CAA96739.1; AAS56807.1; DAA08063.1 

Protein Name

 Nicotinamidase 

Protein Synonyms/Alias

 Nicotine deamidase; NAMase 

Gene Name

 PNC1 

Gene Synonyms/Alias

 YGL037C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
122	KHIKIVDKGFLTDRE	ubiquitination	[1]
147	FHKTDMNKYLEKHHT	acetylation	[2]
207	NKVKEELKAHNINVV	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Catalyzes the deamidation of nicotinamide, an early step in the NAD(+) salvage pathway. Positively regulates SIR2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide, an inhibitor of SIR2, during times of stress. Acts also on nicotinyl hydroxamate. 

Sequence Annotation

 ACT_SITE 8 8
 ACT_SITE 122 122
 ACT_SITE 167 167
 METAL 51 51 Zinc.
 METAL 53 53 Zinc.
 METAL 94 94 Zinc.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Metal-binding; Nucleus; Peroxisome; Pyridine nucleotide biosynthesis; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 216 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005777; C:peroxisome; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008936; F:nicotinamidase activity; IMP:SGD.
 GO:0000183; P:chromatin silencing at rDNA; IMP:SGD.
 GO:0006348; P:chromatin silencing at telomere; IMP:SGD.
 GO:0019358; P:nicotinate nucleotide salvage; ISS:SGD.
 GO:0001302; P:replicative cell aging; IEP:SGD. 

Interpro

 IPR000868; Isochorismatase-like. 

Pfam

 PF00857; Isochorismatase 

SMART

  

PROSITE

  

PRINTS