CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023544
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vesicle-associated membrane protein-associated protein A 
Protein Synonyms/Alias
 VAMP-A; VAMP-associated protein A; VAP-A; 33 kDa VAMP-associated protein; VAP-33 
Gene Name
 Vapa 
Gene Synonyms/Alias
 Vap33 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
24LDPPSDLKFKGPFTDacetylation[1, 2]
26PPSDLKFKGPFTDVVacetylation[2]
125PDELMDSKLRCVFEMacetylation[1, 3]
146LNDMEPSKAVPLNASubiquitination[4]
154AVPLNASKQDGPLPKubiquitination[4]
161KQDGPLPKPHSVSLNacetylation[3]
205DEGLRLRKVAHSDKPubiquitination[4]
211RKVAHSDKPGSTSAVubiquitination[4]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 May play a role in vesicle trafficking. 
Sequence Annotation
 DOMAIN 14 131 MSP.
 MOD_RES 125 125 N6-acetyllysine (By similarity).  
Keyword
 3D-structure; Acetylation; Coiled coil; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Membrane; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 249 AA 
Protein Sequence
MASASGAMAK HEQILVLDPP SDLKFKGPFT DVVTTNLKLQ NPSDRKVCFK VKTTAPRRYC 60
VRPNSGIIDP GSIVTVSVML QPFDYDPNEK SKHKFMVQTI FAPPNISDME AVWKEAKPDE 120
LMDSKLRCVF EMPNENDKLN DMEPSKAVPL NASKQDGPLP KPHSVSLNDT ETRKLMEECK 180
RLQGEMMKLS EENRHLRDEG LRLRKVAHSD KPGSTSAVSF RDNVTSPLPS LLVVIAAIFI 240
GFFLGKFIL 249 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:MGI.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IDA:MGI.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0005923; C:tight junction; IEA:Compara.
 GO:0031982; C:vesicle; IEA:Compara.
 GO:0004871; F:signal transducer activity; IEA:Compara.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0008219; P:cell death; ISS:UniProtKB.
 GO:0031175; P:neuron projection development; ISS:UniProtKB.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IEA:Compara.
 GO:0070972; P:protein localization to endoplasmic reticulum; ISS:UniProtKB. 
Interpro
 IPR000535; Major_sperm.
 IPR008962; PapD-like.
 IPR016763; Vesicle-associated_membrane. 
Pfam
 PF00635; Motile_Sperm 
SMART
  
PROSITE
 PS50202; MSP 
PRINTS