| Tag | Content |
|---|
| CPLM ID | CPLM-016740 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ubiquitin carboxyl-terminal hydrolase 20 |
Protein Synonyms/Alias | Deubiquitinating enzyme 20; Ubiquitin thioesterase 20; Ubiquitin-specific-processing protease 20; VHL-interacting deubiquitinating enzyme 2 |
Gene Name | Usp20 |
Gene Synonyms/Alias | Kiaa1003; Vdu2 |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 908 | ESLHGEQKIEAETRA | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity). |
Sequence Annotation | DOMAIN 689 782 DUSP 1.
DOMAIN 791 894 DUSP 2.
ZN_FING 28 92 UBP-type.
ACT_SITE 154 154 Nucleophile (By similarity).
ACT_SITE 645 645 Proton acceptor (By similarity).
MOD_RES 132 132 Phosphoserine (By similarity).
MOD_RES 134 134 Phosphoserine (By similarity).
MOD_RES 259 259 Phosphothreonine (By similarity).
MOD_RES 378 378 Phosphothreonine.
MOD_RES 415 415 Phosphoserine. |
Keyword | Complete proteome; Cytoplasm; Cytoskeleton; Endocytosis; Hydrolase; Metal-binding; Phosphoprotein; Protease; Reference proteome; Repeat; Thiol protease; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 916 AA |
Protein Sequence | MGDARDLCPH LDCIGEVTKE DLLLKSKGTC QSCGVAGPNL WACLQVTCPY VGCGESFADH 60
SSIHAQVKKH NLTVNLTTFR VWCYACEREV FLEQRLAVHL ASSSARLSEQ DSPPPSHPLK 120
AVPIAVADEG ESESEDDDLK PRGLTGMKNL GNSCYMNAAL QALSNCPPLT QFFLECGGLV 180
RTDKKPALCK SYQKLISEVW HKKRPSYVVP TSLSHGIKLV NPMFRGYAQQ DTQEFLRCLM 240
DQLHEELKEP MVAAVAALTD ARDSDSSDTD ERRDGDRSPS EDEFLSCDSS SDRGEGDGQG 300
RGGGSSKAEM ELLISDEAGR AISEKERMKD RKFSWGQQRT NSEQVDEDAD VDTAMASLDE 360
QSREAQPPSP RSTSPCQTPE PDNEAHIRSS SRPCSPVHHH HEGHSKLSSS PPRASPVRMG 420
PSYVLKKAQV PSTGGRRRKE QSYRSVISDV FNGSVLSLVQ CLTCDRVSTT VETFQDLSLP 480
IPGKEDLAKL HSAIYQNVPA KPGACGDSYS SQGWLAFIVE YIRRFVVSCT PSWFWGPVVT 540
LEDCLAAFFA ADELKGDNMY SCERCKKLRN GVKYCKVLCL PEILCVHLKR FRHEVMYSFK 600
VSSHVSFPLE GLDLRPFLAK ECTSQVTTYD LLSVICHHGT AGSGHYIAYC QNVINGQWYE 660
FDDQYVTEVH ETVVQNVEAY VLFYRKSSEE AMRERQQVVS LAAMREPSLL RFYVSREWLN 720
KFNTFAEPGP ITNHTFLCSH GGIPPNKYHY IDDLVVILPQ SVWEHLYSRF GGGPAVNHLY 780
VCSICQVEIE ALAKRRRVEI DTFIKLNKAF QAEESPAVIY CISMHWFREW EAFVKGKDSE 840
PPGPIDNSRI AQVKGSGHIQ LKQGADCGQI SEETWTYLSS LYGGGPEIAI RQSVAQLPDP 900
ESLHGEQKIE AETRAL 916 |
Gene Ontology | GO:0005813; C:centrosome; ISS:UniProtKB. GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell. GO:0004197; F:cysteine-type endopeptidase activity; ISS:UniProtKB. GO:0004221; F:ubiquitin thiolesterase activity; ISS:UniProtKB. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0006897; P:endocytosis; IEA:UniProtKB-KW. GO:0071108; P:protein K48-linked deubiquitination; ISS:UniProtKB. GO:0070536; P:protein K63-linked deubiquitination; ISS:UniProtKB. GO:0008277; P:regulation of G-protein coupled receptor protein signaling pathway; ISS:UniProtKB. GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |