UniProt Accession

 E7ESA7_HUMAN; E7ESA7 

Genbank Protein ID

 AC010973 

Genbank Nucleotide ID

  

Protein Name

 Nitric oxide synthase, endothelial 

Protein Synonyms/Alias

  

Gene Name

 NOS3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
567	VENLQSSKSTRATIL	ubiquitination	[1]
628	VAVEQLEKGSPGGPP	ubiquitination	[1, 2]
698	PRRYEEWKWFRCPTL	ubiquitination	[2]
829	RLHDIESKGLQPTPM	ubiquitination	[2]
879	SREPDNPKTYVQDIL	ubiquitination	[1, 2, 3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 997 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0010181; F:FMN binding; IEA:InterPro.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0004517; F:nitric-oxide synthase activity; IEA:Compara.
 GO:0001525; P:angiogenesis; IEA:Compara.
 GO:0001974; P:blood vessel remodeling; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0031663; P:lipopolysaccharide-mediated signaling pathway; IEA:Compara.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0051926; P:negative regulation of calcium ion transport; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0051346; P:negative regulation of hydrolase activity; IEA:Compara.
 GO:0014740; P:negative regulation of muscle hyperplasia; IEA:Compara.
 GO:0043267; P:negative regulation of potassium ion transport; IEA:Compara.
 GO:0006809; P:nitric oxide biosynthetic process; IEA:Compara.
 GO:0001542; P:ovulation from ovarian follicle; IEA:Compara.
 GO:0045766; P:positive regulation of angiogenesis; IEA:Compara.
 GO:0050880; P:regulation of blood vessel size; IEA:Compara.
 GO:0002028; P:regulation of sodium ion transport; IEA:Compara.
 GO:0003057; P:regulation of the force of heart contraction by chemical signal; IEA:Compara.
 GO:0014806; P:smooth muscle hyperplasia; IEA:Compara. 

Interpro

 IPR003097; FAD-binding_1.
 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001094; Flavdoxin.
 IPR008254; Flavodoxin/NO_synth.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR023173; NADPH_Cyt_P450_Rdtase_dom3.
 IPR004030; NO_synthase_oxygenase_dom.
 IPR026009; NOS.
 IPR012144; NOS_met.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR017938; Riboflavin_synthase-like_b-brl. 

Pfam

 PF00667; FAD_binding_1
 PF00258; Flavodoxin_1
 PF00175; NAD_binding_1
 PF02898; NO_synthase 

SMART

  

PROSITE

 PS51384; FAD_FR
 PS50902; FLAVODOXIN_LIKE 

PRINTS

 PR00369; FLAVODOXIN.
 PR00371; FPNCR.