CPLM-015643

Genbank Nucleotide ID

Mediator of RNA polymerase II transcription subunit 25

Protein Synonyms/Alias

Activator interaction domain-containing protein 1; Activator-recruited cofactor 92 kDa component; ARC92; Mediator complex subunit 25; p78

ACID1; ARC92; PTOV2; TCBAP0758

Homo sapiens (Human)

Position	Peptide	Type	References
197	ALRLLFEKAAPPALL	ubiquitination	[1, 2]
484	NKDLESLKGLYRIMG	ubiquitination	[3]
551	HKQVQQQKLEQQQRG	ubiquitination	[4, 5]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.

REGION 1 226 Interaction with the Mediator complex.
REGION 389 543 Interaction with VP16.
REGION 395 545 Interaction with CREBBP.
REGION 564 653 Interaction with RARA.
REGION 640 707 Interaction with RARA.
MOTIF 646 650 LXXLL motif.

3D-structure; Activator; Alternative splicing; Charcot-Marie-Tooth disease; Complete proteome; Direct protein sequencing; Disease mutation; Neuropathy; Nucleus; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0048147; P:negative regulation of fibroblast proliferation; IMP:UniProtKB.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
GO:0071158; P:positive regulation of cell cycle arrest; IMP:UniProtKB.
GO:0035563; P:positive regulation of chromatin binding; IMP:UniProtKB.
GO:2001178; P:positive regulation of mediator complex assembly; IMP:UniProtKB.
GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.

IPR021394; Mediator_Med25.
IPR021406; Mediator_Med25_NR-box.
IPR021397; Mediator_Med25_SD1.
IPR021419; Mediator_Med25_VWA.

PF11232; Med25
PF11244; Med25_NR-box
PF11235; Med25_SD1
PF11265; Med25_VWA