UniProt Accession

 METK_ECOLI; P0A817; P04384; P30869; Q2M9Q1 

Genbank Protein ID

 K02129; U28377; U00096; AP009048; M98266; M31770 

Genbank Nucleotide ID

 AAA24164.1; AAA69109.1; AAC75979.1; BAE77005.1; AAB05197.1; AAA24645.2 

Protein Name

 S-adenosylmethionine synthase 

Protein Synonyms/Alias

 AdoMet synthase; MAT; Methionine adenosyltransferase 

Gene Name

 metK 

Gene Synonyms/Alias

 metX; b2942; JW2909 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
3	*****MAKHLFTSES	acetylation	[1, 2]
37	AILEQDPKARVACET	acetylation	[3]
166	PWLRPDAKSQVTFQY	acetylation	[3]
209	AVMEEIIKPILPAEW	acetylation	[3]
246	DCGLTGRKIIVDTYG	acetylation	[3]
266	GGGAFSGKDPSKVDR	acetylation	[3]
270	FSGKDPSKVDRSAAY	acetylation	[3]
284	YAARYVAKNIVAAGL	acetylation	[3]
370	REHFPWEKTDKAQLL	acetylation	[3]
373	FPWEKTDKAQLLRDA	acetylation	[3]

Reference

 [1] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. Is essential for growth. 

Sequence Annotation

 NP_BIND 260 267 ATP (Potential).
 REGION 239 248 Pyrophosphate binding.
 METAL 17 17 Magnesium.
 METAL 43 43 Potassium.
 METAL 264 264 Potassium.
 METAL 272 272 Magnesium.
 BINDING 119 119 Methionine.
 BINDING 239 239 Methionine.
 MOD_RES 3 3 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Cobalt; Complete proteome; Cytoplasm; Magnesium; Metal-binding; Nucleotide-binding; One-carbon metabolism; Potassium; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 384 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IDA:EcoCyc.
 GO:0004478; F:methionine adenosyltransferase activity; IDA:EcoCyc.
 GO:0030955; F:potassium ion binding; IDA:EcoCyc.
 GO:0006730; P:one-carbon metabolic process; IEA:HAMAP.
 GO:0006556; P:S-adenosylmethionine biosynthetic process; IMP:EcoCyc. 

Interpro

 IPR022631; ADOMET_SYNTHASE_CS.
 IPR022630; S-AdoMet_synt_C.
 IPR022629; S-AdoMet_synt_central.
 IPR022628; S-AdoMet_synt_N.
 IPR002133; S-AdoMet_synthetase.
 IPR022636; S-AdoMet_synthetase_sfam. 

Pfam

 PF02773; S-AdoMet_synt_C
 PF02772; S-AdoMet_synt_M
 PF00438; S-AdoMet_synt_N 

SMART

  

PROSITE

 PS00376; ADOMET_SYNTHASE_1
 PS00377; ADOMET_SYNTHASE_2 

PRINTS