UniProt Accession

 CASP3_MOUSE; P70677; O08668; Q8CHV5; Q9QWI4 

Genbank Protein ID

 U54803; U54802; U49929; D86352; Y13086; U19522; BC038825; U63720 

Genbank Nucleotide ID

 AAC52768.1; AAC52768.1; AAC52764.1; BAA21727.1; CAA73528.1; AAC53196.1; AAH38825.2; AAD09504.1 

Protein Name

 Caspase-3 

Protein Synonyms/Alias

 CASP-3; Apopain; Cysteine protease CPP32; CPP-32; LICE; Protein Yama; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12 

Gene Name

 Casp3 

Gene Synonyms/Alias

 Cpp32 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
11	NKTSVDSKSINNFEV	acetylation	[1, 2, 3]
19	SINNFEVKTIHGSKS	acetylation	[1]
19	SINNFEVKTIHGSKS	ubiquitination	[4]
25	VKTIHGSKSVDSGIY	acetylation	[1, 3]
25	VKTIHGSKSVDSGIY	ubiquitination	[4]
57	INNKNFHKSTGMSSR	ubiquitination	[4]
82	RETFMGLKYQVRNKN	acetylation	[1]
82	RETFMGLKYQVRNKN	ubiquitination	[4]
154	YCRSLTGKPKLFIIQ	acetylation	[1]
156	RSLTGKPKLFIIQAC	acetylation	[1]
229	MLKLYAHKLEFMHIL	acetylation	[1]
260	DSTFHAKKQIPCIVS	acetylation	[1]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. 

Sequence Annotation

 ACT_SITE 121 121 By similarity.
 ACT_SITE 163 163 By similarity.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 26 26 Phosphoserine.
 MOD_RES 163 163 S-nitrosocysteine; in inhibited form (By  

Keyword

 Acetylation; Apoptosis; Complete proteome; Cytoplasm; Hydrolase; Phosphoprotein; Protease; Reference proteome; S-nitrosylation; Thiol protease; Zymogen. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 277 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0004190; F:aspartic-type endopeptidase activity; IDA:MGI.
 GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; IMP:MGI.
 GO:0097200; F:cysteine-type endopeptidase activity involved in execution phase of apoptosis; IDA:MGI.
 GO:0006309; P:apoptotic DNA fragmentation; IMP:MGI.
 GO:0001782; P:B cell homeostasis; IMP:MGI.
 GO:0045165; P:cell fate commitment; IMP:MGI.
 GO:0071310; P:cellular response to organic substance; IDA:MGI.
 GO:0097192; P:extrinsic apoptotic signaling pathway in absence of ligand; IDA:MGI.
 GO:0008625; P:extrinsic apoptotic signaling pathway via death domain receptors; IMP:MGI.
 GO:0034349; P:glial cell apoptotic process; IMP:MGI.
 GO:0007507; P:heart development; IGI:MGI.
 GO:0006917; P:induction of apoptosis; IDA:MGI.
 GO:0008631; P:intrinsic apoptotic signaling pathway in response to oxidative stress; IDA:MGI.
 GO:0030216; P:keratinocyte differentiation; IMP:MGI.
 GO:0046007; P:negative regulation of activated T cell proliferation; IMP:MGI.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0030889; P:negative regulation of B cell proliferation; IMP:MGI.
 GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; IMP:MGI.
 GO:0051402; P:neuron apoptotic process; IDA:MGI.
 GO:0030264; P:nuclear fragmentation involved in apoptotic nuclear change; IEA:Compara.
 GO:0043525; P:positive regulation of neuron apoptotic process; IDA:MGI.
 GO:0016485; P:protein processing; IDA:MGI.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0001836; P:release of cytochrome c from mitochondria; IGI:MGI.
 GO:0006974; P:response to DNA damage stimulus; IDA:MGI.
 GO:0009411; P:response to UV; IDA:MGI.
 GO:0009611; P:response to wounding; IDA:MGI.
 GO:0007605; P:sensory perception of sound; IMP:MGI.
 GO:0043029; P:T cell homeostasis; IMP:MGI. 

Interpro

 IPR015470; Caspase_3.
 IPR011600; Pept_C14_cat.
 IPR001309; Pept_C14_ICE_p20.
 IPR016129; Pept_C14_ICE_p20_AS.
 IPR002138; Pept_C14_p10.
 IPR002398; Pept_C14_p45.
 IPR015917; Pept_C14_p45_core. 

Pfam

 PF00656; Peptidase_C14 

SMART

 SM00115; CASc 

PROSITE

 PS01122; CASPASE_CYS
 PS01121; CASPASE_HIS
 PS50207; CASPASE_P10
 PS50208; CASPASE_P20 

PRINTS

 PR00376; IL1BCENZYME.