CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019732
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear pore complex protein Nup88 
Protein Synonyms/Alias
 88 kDa nucleoporin; Nucleoporin Nup88 
Gene Name
 NUP88 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
41QSPTEAEKPASSSLPubiquitination[1, 2]
138LMVLELPKRWGKNSEubiquitination[2]
142ELPKRWGKNSEFEGGubiquitination[1, 2, 3]
150NSEFEGGKSTVNCSTubiquitination[1, 2, 4]
226EESLVLNKGRAYTASubiquitination[1, 2, 3, 5]
394SDFSCPVKLHRDPKCubiquitination[2]
435LGSDEEDKDSLQELSubiquitination[1, 2]
456VEHILCTKPLPCRQPubiquitination[2]
531ETPDSFEKHIRSILQubiquitination[1, 2]
548VANPAFLKASEKDIAubiquitination[1, 2, 3]
552AFLKASEKDIAPPPEubiquitination[2]
579FREQYILKQDLAKEEubiquitination[1, 2]
584ILKQDLAKEEIQRRVubiquitination[2]
600LLCDQKKKQLEDLSYubiquitination[2]
625MAERLADKYEEAKEKubiquitination[1]
630ADKYEEAKEKQEDIMubiquitination[1]
642DIMNRMKKLLHSFHSubiquitination[2]
663DSERDMKKELQLIPDubiquitination[2]
680RHLGNAIKQVTMKKDubiquitination[1, 2, 6]
692KKDYQQQKMEKVLSLubiquitination[1, 2]
695YQQQKMEKVLSLPKPubiquitination[2]
712ILSAYQRKCIQSILKubiquitination[1, 2]
719KCIQSILKEEGEHIRubiquitination[1, 2]
730EHIREMVKQINDIRNubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Essential component of nuclear pore complex. 
Sequence Annotation
 MOD_RES 35 35 Phosphoserine.
 MOD_RES 50 50 Phosphoserine.
 MOD_RES 517 517 Phosphoserine.
 MOD_RES 525 525 Phosphothreonine.
 MOD_RES 540 540 Phosphoserine.  
Keyword
 Coiled coil; Complete proteome; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 741 AA 
Protein Sequence
MAAAEGPVGD GELWQTWLPN HVVFLRLREG LKNQSPTEAE KPASSSLPSS PPPQLLTRNV 60
VFGLGGELFL WDGEDSSFLV VRLRGPSGGG EEPALSQYQR LLCINPPLFE IYQVLLSPTQ 120
HHVALIGIKG LMVLELPKRW GKNSEFEGGK STVNCSTTPV AERFFTSSTS LTLKHAAWYP 180
SEILDPHVVL LTSDNVIRIY SLREPQTPTN VIILSEAEEE SLVLNKGRAY TASLGETAVA 240
FDFGPLAAVP KTLFGQNGKD EVVAYPLYIL YENGETFLTY ISLLHSPGNI GKLLGPLPMH 300
PAAEDNYGYD ACAVLCLPCV PNILVIATES GMLYHCVVLE GEEEDDHTSE KSWDSRIDLI 360
PSLYVFECVE LELALKLASG EDDPFDSDFS CPVKLHRDPK CPSRYHCTHE AGVHSVGLTW 420
IHKLHKFLGS DEEDKDSLQE LSTEQKCFVE HILCTKPLPC RQPAPIRGFW IVPDILGPTM 480
ICITSTYECL IWPLLSTVHP ASPPLLCTRE DVEVAESPLR VLAETPDSFE KHIRSILQRS 540
VANPAFLKAS EKDIAPPPEE CLQLLSRATQ VFREQYILKQ DLAKEEIQRR VKLLCDQKKK 600
QLEDLSYCRE ERKSLREMAE RLADKYEEAK EKQEDIMNRM KKLLHSFHSE LPVLSDSERD 660
MKKELQLIPD QLRHLGNAIK QVTMKKDYQQ QKMEKVLSLP KPTIILSAYQ RKCIQSILKE 720
EGEHIREMVK QINDIRNHVN F 741 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005643; C:nuclear pore; TAS:ProtInc.
 GO:0005215; F:transporter activity; TAS:ProtInc.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0015758; P:glucose transport; TAS:Reactome.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0010827; P:regulation of glucose transport; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR019321; Nucleoporin_Nup88. 
Pfam
 PF10168; Nup88 
SMART
  
PROSITE
  
PRINTS