CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023061
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ADP-ribosylation factor-binding protein GGA1 
Protein Synonyms/Alias
 Gamma-adaptin-related protein 1; Golgi-localized, gamma ear-containing, ARF-binding protein 1 
Gene Name
 GGA1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
54ATRLLAHKIQSPQEWubiquitination[1]
101LIKVVSPKYLGSRTSubiquitination[2]
141EAYQMLKKQGIVKSDubiquitination[1]
165PLPPPRPKNVIFEDEubiquitination[1, 2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. 
Sequence Annotation
 DOMAIN 17 147 VHS.
 DOMAIN 171 299 GAT.
 DOMAIN 510 631 GAE.
 REGION 114 274 Interaction with ARF3.
 REGION 300 509 Unstructured hinge.
 MOTIF 358 362 Autoinhibitory.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 355 355 Phosphoserine; by CK2 (Probable).  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Endosome; Golgi apparatus; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 639 AA 
Protein Sequence
MEPAMEPETL EARINRATNP LNKELDWASI NGFCEQLNED FEGPPLATRL LAHKIQSPQE 60
WEAIQALTVL ETCMKSCGKR FHDEVGKFRF LNELIKVVSP KYLGSRTSEK VKNKILELLY 120
SWTVGLPEEV KIAEAYQMLK KQGIVKSDPK LPDDTTFPLP PPRPKNVIFE DEEKSKMLAR 180
LLKSSHPEDL RAANKLIKEM VQEDQKRMEK ISKRVNAIEE VNNNVKLLTE MVMSHSQGGA 240
AAGSSEDLMK ELYQRCERMR PTLFRLASDT EDNDEALAEI LQANDNLTQV INLYKQLVRG 300
EEVNGDATAG SIPGSTSALL DLSGLDLPPA GTTYPAMPTR PGEQASPEQP SASVSLLDDE 360
LMSLGLSDPT PPSGPSLDGT GWNSFQSSDA TEPPAPALAQ APSMESRPPA QTSLPASSGL 420
DDLDLLGKTL LQQSLPPESQ QVRWEKQQPT PRLTLRDLQN KSSSCSSPSS SATSLLHTVS 480
PEPPRPPQQP VPTELSLASI TVPLESIKPS NILPVTVYDQ HGFRILFHFA RDPLPGRSDV 540
LVVVVSMLST APQPIRNIVF QSAVPKVMKV KLQPPSGTEL PAFNPIVHPS AITQVLLLAN 600
PQKEKVRLRY KLTFTMGDQT YNEMGDVDQF PPPETWGSL 639 
Gene Ontology
 GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; TAS:UniProtKB.
 GO:0006886; P:intracellular protein transport; TAS:UniProtKB.
 GO:0016192; P:vesicle-mediated transport; IEA:InterPro. 
Interpro
 IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
 IPR008153; Clathrin_g-adaptin_app.
 IPR013041; Coatomer/clathrin_app_Ig-like.
 IPR008942; ENTH_VHS.
 IPR004152; GAT.
 IPR002014; VHS.
 IPR018205; VHS_subgr. 
Pfam
 PF02883; Alpha_adaptinC2
 PF03127; GAT
 PF00790; VHS 
SMART
 SM00809; Alpha_adaptinC2
 SM00288; VHS 
PROSITE
 PS50180; GAE
 PS50909; GAT
 PS50179; VHS 
PRINTS